Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway

Hepatocellular carcinoma (HCC) is one of the common malignances in the world and has high mortality in part due to development of acquired drug resistance. Therefore, it is urgent to investigate the molecular mechanism of drug resistance in HCC. To explore the underlying mechanism of drug resistance...

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Autores principales: Wu, Qiong, Wang, Rui, Yang, Qingling, Hou, Xin, Chen, Sulian, Hou, Yueyue, Chen, Changjie, Yang, Yan, Miele, Lucio, Sarkar, Fazlul H, Chen, Yuqing, Wang, Zhiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875765/
https://www.ncbi.nlm.nih.gov/pubmed/24158561
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author Wu, Qiong
Wang, Rui
Yang, Qingling
Hou, Xin
Chen, Sulian
Hou, Yueyue
Chen, Changjie
Yang, Yan
Miele, Lucio
Sarkar, Fazlul H
Chen, Yuqing
Wang, Zhiwei
author_facet Wu, Qiong
Wang, Rui
Yang, Qingling
Hou, Xin
Chen, Sulian
Hou, Yueyue
Chen, Changjie
Yang, Yan
Miele, Lucio
Sarkar, Fazlul H
Chen, Yuqing
Wang, Zhiwei
author_sort Wu, Qiong
collection PubMed
description Hepatocellular carcinoma (HCC) is one of the common malignances in the world and has high mortality in part due to development of acquired drug resistance. Therefore, it is urgent to investigate the molecular mechanism of drug resistance in HCC. To explore the underlying mechanism of drug resistance in HCC, we developed gemcitabine-resistant (GR) HCC cells. We used multiple methods to achieve our goal including RT-PCR, Western blotting analysis, transfection, Wound-healing assay, migration and invasion assay. We observed that gemcitabine-resistant cells acquired epithelial-mesenchymal transition (EMT) phenotype. Moreover, we found that PDGF-D is highly expressed in GR cells. Furthermore, down-regulation of PDGF-D in GR cells led to partial reversal of the EMT phenotype. Our findings demonstrated that targeting PDGF-D could be a novel strategy to overcome gemcitabine resistance in HCC.
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spelling pubmed-38757652014-01-07 Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway Wu, Qiong Wang, Rui Yang, Qingling Hou, Xin Chen, Sulian Hou, Yueyue Chen, Changjie Yang, Yan Miele, Lucio Sarkar, Fazlul H Chen, Yuqing Wang, Zhiwei Oncotarget Research Paper Hepatocellular carcinoma (HCC) is one of the common malignances in the world and has high mortality in part due to development of acquired drug resistance. Therefore, it is urgent to investigate the molecular mechanism of drug resistance in HCC. To explore the underlying mechanism of drug resistance in HCC, we developed gemcitabine-resistant (GR) HCC cells. We used multiple methods to achieve our goal including RT-PCR, Western blotting analysis, transfection, Wound-healing assay, migration and invasion assay. We observed that gemcitabine-resistant cells acquired epithelial-mesenchymal transition (EMT) phenotype. Moreover, we found that PDGF-D is highly expressed in GR cells. Furthermore, down-regulation of PDGF-D in GR cells led to partial reversal of the EMT phenotype. Our findings demonstrated that targeting PDGF-D could be a novel strategy to overcome gemcitabine resistance in HCC. Impact Journals LLC 2013-10-07 /pmc/articles/PMC3875765/ /pubmed/24158561 Text en Copyright: © 2013 Wu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Paper
Wu, Qiong
Wang, Rui
Yang, Qingling
Hou, Xin
Chen, Sulian
Hou, Yueyue
Chen, Changjie
Yang, Yan
Miele, Lucio
Sarkar, Fazlul H
Chen, Yuqing
Wang, Zhiwei
Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway
title Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway
title_full Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway
title_fullStr Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway
title_full_unstemmed Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway
title_short Chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of PDGF-D pathway
title_sort chemoresistance to gemcitabine in hepatoma cells induces epithelial-mesenchymal transition and involves activation of pdgf-d pathway
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875765/
https://www.ncbi.nlm.nih.gov/pubmed/24158561
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