Cargando…

Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial

Reactive oxygen species are important risk factors for age-related diseases, but they also act as signaling factors for endogenous antioxidative defense. The hypothesis that a multi-micronutrient supplement with nutritional doses of antioxidant nutrients and phytochemicals (MP) may provide protectio...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, You Jin, Ahn, Yoon Hee, Lim, Yeni, Kim, Ji Yeon, Kim, Joohee, Kwon, Oran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875922/
https://www.ncbi.nlm.nih.gov/pubmed/24352096
http://dx.doi.org/10.3390/nu5125218
_version_ 1782297431015161856
author Kim, You Jin
Ahn, Yoon Hee
Lim, Yeni
Kim, Ji Yeon
Kim, Joohee
Kwon, Oran
author_facet Kim, You Jin
Ahn, Yoon Hee
Lim, Yeni
Kim, Ji Yeon
Kim, Joohee
Kwon, Oran
author_sort Kim, You Jin
collection PubMed
description Reactive oxygen species are important risk factors for age-related diseases, but they also act as signaling factors for endogenous antioxidative defense. The hypothesis that a multi-micronutrient supplement with nutritional doses of antioxidant nutrients and phytochemicals (MP) may provide protection against oxidative damage and maintain the endogenous antioxidant defense capacity was assessed in subjects with a habitually low intake of fruits and vegetables. In a randomized, placebo-controlled, and parallel designed trial, 89 eligible subjects were assigned to either placebo or MP for eight weeks. Eighty subjects have completed the protocol and included for the analysis. MP treatment was superior at increasing serum folate (p < 0.0001) and resistance to DNA damage (p = 0.006, tail intensity; p = 0.030, tail moment by comet assay), and LDL oxidation (p = 0.009) compared with the placebo. Moreover, the endogenous oxidative defense capacity was not weakened after MP supplementation, as determined by the levels of glutathione peroxidase (p = 0.442), catalase (p = 0.686), and superoxide dismutase (p = 0.804). The serum folate level was negatively correlated with DNA damage (r = −0.376, p = 0.001 for tail density; r = −0.329, p = 0.003 for tail moment), but no correlation was found with LDL oxidation (r = −0.123, p = 0.275). These results suggest that MP use in healthy subjects with habitually low dietary fruit and vegetable intake may be beneficial in providing resistance to oxidative damage to DNA and LDL without suppressing the endogenous defense mechanisms.
format Online
Article
Text
id pubmed-3875922
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-38759222013-12-31 Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial Kim, You Jin Ahn, Yoon Hee Lim, Yeni Kim, Ji Yeon Kim, Joohee Kwon, Oran Nutrients Article Reactive oxygen species are important risk factors for age-related diseases, but they also act as signaling factors for endogenous antioxidative defense. The hypothesis that a multi-micronutrient supplement with nutritional doses of antioxidant nutrients and phytochemicals (MP) may provide protection against oxidative damage and maintain the endogenous antioxidant defense capacity was assessed in subjects with a habitually low intake of fruits and vegetables. In a randomized, placebo-controlled, and parallel designed trial, 89 eligible subjects were assigned to either placebo or MP for eight weeks. Eighty subjects have completed the protocol and included for the analysis. MP treatment was superior at increasing serum folate (p < 0.0001) and resistance to DNA damage (p = 0.006, tail intensity; p = 0.030, tail moment by comet assay), and LDL oxidation (p = 0.009) compared with the placebo. Moreover, the endogenous oxidative defense capacity was not weakened after MP supplementation, as determined by the levels of glutathione peroxidase (p = 0.442), catalase (p = 0.686), and superoxide dismutase (p = 0.804). The serum folate level was negatively correlated with DNA damage (r = −0.376, p = 0.001 for tail density; r = −0.329, p = 0.003 for tail moment), but no correlation was found with LDL oxidation (r = −0.123, p = 0.275). These results suggest that MP use in healthy subjects with habitually low dietary fruit and vegetable intake may be beneficial in providing resistance to oxidative damage to DNA and LDL without suppressing the endogenous defense mechanisms. MDPI 2013-12-18 /pmc/articles/PMC3875922/ /pubmed/24352096 http://dx.doi.org/10.3390/nu5125218 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kim, You Jin
Ahn, Yoon Hee
Lim, Yeni
Kim, Ji Yeon
Kim, Joohee
Kwon, Oran
Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial
title Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial
title_full Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial
title_fullStr Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial
title_full_unstemmed Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial
title_short Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial
title_sort daily nutritional dose supplementation with antioxidant nutrients and phytochemicals improves dna and ldl stability: a double-blind, randomized, and placebo-controlled trial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875922/
https://www.ncbi.nlm.nih.gov/pubmed/24352096
http://dx.doi.org/10.3390/nu5125218
work_keys_str_mv AT kimyoujin dailynutritionaldosesupplementationwithantioxidantnutrientsandphytochemicalsimprovesdnaandldlstabilityadoubleblindrandomizedandplacebocontrolledtrial
AT ahnyoonhee dailynutritionaldosesupplementationwithantioxidantnutrientsandphytochemicalsimprovesdnaandldlstabilityadoubleblindrandomizedandplacebocontrolledtrial
AT limyeni dailynutritionaldosesupplementationwithantioxidantnutrientsandphytochemicalsimprovesdnaandldlstabilityadoubleblindrandomizedandplacebocontrolledtrial
AT kimjiyeon dailynutritionaldosesupplementationwithantioxidantnutrientsandphytochemicalsimprovesdnaandldlstabilityadoubleblindrandomizedandplacebocontrolledtrial
AT kimjoohee dailynutritionaldosesupplementationwithantioxidantnutrientsandphytochemicalsimprovesdnaandldlstabilityadoubleblindrandomizedandplacebocontrolledtrial
AT kwonoran dailynutritionaldosesupplementationwithantioxidantnutrientsandphytochemicalsimprovesdnaandldlstabilityadoubleblindrandomizedandplacebocontrolledtrial