Cbl-b Enhances Sensitivity to 5-Fluorouracil via EGFR- and Mitochondria-Mediated Pathways in Gastric Cancer Cells

5-Fluorouracil (5-FU) is an essential component of anticancer chemotherapy against gastric cancer. However, the response rate of single drug is still limited. The ubiquitin ligase Cbl-b is a negative regulator of growth factor receptor signaling and is involved in the suppression of cancer cell prol...

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Autores principales: Feng, Dan, Ma, Yanju, Liu, Jing, Xu, Ling, Zhang, Ye, Qu, Jinglei, Liu, Yunpeng, Qu, Xiujuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876118/
https://www.ncbi.nlm.nih.gov/pubmed/24351824
http://dx.doi.org/10.3390/ijms141224399
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author Feng, Dan
Ma, Yanju
Liu, Jing
Xu, Ling
Zhang, Ye
Qu, Jinglei
Liu, Yunpeng
Qu, Xiujuan
author_facet Feng, Dan
Ma, Yanju
Liu, Jing
Xu, Ling
Zhang, Ye
Qu, Jinglei
Liu, Yunpeng
Qu, Xiujuan
author_sort Feng, Dan
collection PubMed
description 5-Fluorouracil (5-FU) is an essential component of anticancer chemotherapy against gastric cancer. However, the response rate of single drug is still limited. The ubiquitin ligase Cbl-b is a negative regulator of growth factor receptor signaling and is involved in the suppression of cancer cell proliferation. However, whether Cbl-b could affect 5-FU sensitivity remains unclear. The present study showed that Cbl-b knockdown caused higher proliferation concomitant with the decrease of apoptosis induced by 5-FU treatment in gastric cancer cell. Further mechanism investigation demonstrated that Cbl-b knockdown caused significant increase of phosphorylation of EGFR, ERK and Akt, decrease of mitochondrial membrane potential, and increase of expression ratio of Bcl-2/Bax. These results suggest that Cbl-b enhances sensitivity to 5-FU via EGFR- and mitochondria-mediated pathways in gastric cancer cells.
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spelling pubmed-38761182013-12-31 Cbl-b Enhances Sensitivity to 5-Fluorouracil via EGFR- and Mitochondria-Mediated Pathways in Gastric Cancer Cells Feng, Dan Ma, Yanju Liu, Jing Xu, Ling Zhang, Ye Qu, Jinglei Liu, Yunpeng Qu, Xiujuan Int J Mol Sci Article 5-Fluorouracil (5-FU) is an essential component of anticancer chemotherapy against gastric cancer. However, the response rate of single drug is still limited. The ubiquitin ligase Cbl-b is a negative regulator of growth factor receptor signaling and is involved in the suppression of cancer cell proliferation. However, whether Cbl-b could affect 5-FU sensitivity remains unclear. The present study showed that Cbl-b knockdown caused higher proliferation concomitant with the decrease of apoptosis induced by 5-FU treatment in gastric cancer cell. Further mechanism investigation demonstrated that Cbl-b knockdown caused significant increase of phosphorylation of EGFR, ERK and Akt, decrease of mitochondrial membrane potential, and increase of expression ratio of Bcl-2/Bax. These results suggest that Cbl-b enhances sensitivity to 5-FU via EGFR- and mitochondria-mediated pathways in gastric cancer cells. Molecular Diversity Preservation International (MDPI) 2013-12-16 /pmc/articles/PMC3876118/ /pubmed/24351824 http://dx.doi.org/10.3390/ijms141224399 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Feng, Dan
Ma, Yanju
Liu, Jing
Xu, Ling
Zhang, Ye
Qu, Jinglei
Liu, Yunpeng
Qu, Xiujuan
Cbl-b Enhances Sensitivity to 5-Fluorouracil via EGFR- and Mitochondria-Mediated Pathways in Gastric Cancer Cells
title Cbl-b Enhances Sensitivity to 5-Fluorouracil via EGFR- and Mitochondria-Mediated Pathways in Gastric Cancer Cells
title_full Cbl-b Enhances Sensitivity to 5-Fluorouracil via EGFR- and Mitochondria-Mediated Pathways in Gastric Cancer Cells
title_fullStr Cbl-b Enhances Sensitivity to 5-Fluorouracil via EGFR- and Mitochondria-Mediated Pathways in Gastric Cancer Cells
title_full_unstemmed Cbl-b Enhances Sensitivity to 5-Fluorouracil via EGFR- and Mitochondria-Mediated Pathways in Gastric Cancer Cells
title_short Cbl-b Enhances Sensitivity to 5-Fluorouracil via EGFR- and Mitochondria-Mediated Pathways in Gastric Cancer Cells
title_sort cbl-b enhances sensitivity to 5-fluorouracil via egfr- and mitochondria-mediated pathways in gastric cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876118/
https://www.ncbi.nlm.nih.gov/pubmed/24351824
http://dx.doi.org/10.3390/ijms141224399
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