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Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression

Necrostatin-1 (Nec-1) inhibits necroptosis and is usually regarded as having no effect on other cell deaths. Here, this study explored whether the addition of Nec-1 has an effect on cell death induced by simulated ischemia injury in rat tubular cell line NRK-52E. In addition, we also investigated th...

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Autores principales: Zhang, Li, Jiang, Fen, Chen, Yuanhan, Luo, Jialun, Liu, Shuangxin, Zhang, Bin, Ye, Zhiming, Wang, Wenjian, Liang, Xinling, Shi, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876139/
https://www.ncbi.nlm.nih.gov/pubmed/24351845
http://dx.doi.org/10.3390/ijms141224742
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author Zhang, Li
Jiang, Fen
Chen, Yuanhan
Luo, Jialun
Liu, Shuangxin
Zhang, Bin
Ye, Zhiming
Wang, Wenjian
Liang, Xinling
Shi, Wei
author_facet Zhang, Li
Jiang, Fen
Chen, Yuanhan
Luo, Jialun
Liu, Shuangxin
Zhang, Bin
Ye, Zhiming
Wang, Wenjian
Liang, Xinling
Shi, Wei
author_sort Zhang, Li
collection PubMed
description Necrostatin-1 (Nec-1) inhibits necroptosis and is usually regarded as having no effect on other cell deaths. Here, this study explored whether the addition of Nec-1 has an effect on cell death induced by simulated ischemia injury in rat tubular cell line NRK-52E. In addition, we also investigated the mechanism of Nec-1 attenuates cell death in this renal ischemia model. The NRK-52E cells were incubated with TNF-α + antimycinA (TA) for 24 h with or without Nec-1. Cell death was observed under fluorescent microscope and quantified by flow cytometry. Cell viabilities were detected by MTT assay. The protein expression of dynamin-related protein 1 (Drp1) was detected by Western blotting and immunofluorescence assay. Increased cell death in simulated ischemia injury of NRK-52E cells were markedly attenuated in the Nec-1 pretreated ischemia injury group. Meanwhile, cell viability was significantly improved after using Nec-1. In addition, we also observed that the protein expression of Drp1, a mediator of mitochondrial fission, was significantly increased in simulated ischemia injury group. Increased Drp1 expression in the ischemia injury group can be abolished by Nec-1 or Drp1-knock down, accompanied with decreased cell death and improved cell viabilities. These results suggest that Nec-1 may inhibit cell death induced by simulated ischemia injury in the rat tubular cell line NRK-52E through decreased Drp1 expression.
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spelling pubmed-38761392013-12-31 Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression Zhang, Li Jiang, Fen Chen, Yuanhan Luo, Jialun Liu, Shuangxin Zhang, Bin Ye, Zhiming Wang, Wenjian Liang, Xinling Shi, Wei Int J Mol Sci Article Necrostatin-1 (Nec-1) inhibits necroptosis and is usually regarded as having no effect on other cell deaths. Here, this study explored whether the addition of Nec-1 has an effect on cell death induced by simulated ischemia injury in rat tubular cell line NRK-52E. In addition, we also investigated the mechanism of Nec-1 attenuates cell death in this renal ischemia model. The NRK-52E cells were incubated with TNF-α + antimycinA (TA) for 24 h with or without Nec-1. Cell death was observed under fluorescent microscope and quantified by flow cytometry. Cell viabilities were detected by MTT assay. The protein expression of dynamin-related protein 1 (Drp1) was detected by Western blotting and immunofluorescence assay. Increased cell death in simulated ischemia injury of NRK-52E cells were markedly attenuated in the Nec-1 pretreated ischemia injury group. Meanwhile, cell viability was significantly improved after using Nec-1. In addition, we also observed that the protein expression of Drp1, a mediator of mitochondrial fission, was significantly increased in simulated ischemia injury group. Increased Drp1 expression in the ischemia injury group can be abolished by Nec-1 or Drp1-knock down, accompanied with decreased cell death and improved cell viabilities. These results suggest that Nec-1 may inhibit cell death induced by simulated ischemia injury in the rat tubular cell line NRK-52E through decreased Drp1 expression. Molecular Diversity Preservation International (MDPI) 2013-12-18 /pmc/articles/PMC3876139/ /pubmed/24351845 http://dx.doi.org/10.3390/ijms141224742 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Li
Jiang, Fen
Chen, Yuanhan
Luo, Jialun
Liu, Shuangxin
Zhang, Bin
Ye, Zhiming
Wang, Wenjian
Liang, Xinling
Shi, Wei
Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression
title Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression
title_full Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression
title_fullStr Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression
title_full_unstemmed Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression
title_short Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression
title_sort necrostatin-1 attenuates ischemia injury induced cell death in rat tubular cell line nrk-52e through decreased drp1 expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876139/
https://www.ncbi.nlm.nih.gov/pubmed/24351845
http://dx.doi.org/10.3390/ijms141224742
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