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Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System
The periodic expression and destruction of several cyclins are the most important steps for the exact regulation of cell cycle. Cyclins are degraded by the ubiquitin-proteasome system during cell cycle. Besides, a short sequence near the N-terminal of cyclin B called the destruction box (D-box; CDB)...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876668/ https://www.ncbi.nlm.nih.gov/pubmed/24416725 http://dx.doi.org/10.1155/2013/732307 |
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author | Yang, Chao-Hsun Kuo, Wan-Ting Chuang, Yun-Ting Chen, Cheng-Yu Lin, Chih-Chien |
author_facet | Yang, Chao-Hsun Kuo, Wan-Ting Chuang, Yun-Ting Chen, Cheng-Yu Lin, Chih-Chien |
author_sort | Yang, Chao-Hsun |
collection | PubMed |
description | The periodic expression and destruction of several cyclins are the most important steps for the exact regulation of cell cycle. Cyclins are degraded by the ubiquitin-proteasome system during cell cycle. Besides, a short sequence near the N-terminal of cyclin B called the destruction box (D-box; CDB) is also required. Fluorescent-protein-based reporter gene system is insensitive to analysis because of the overly stable fluorescent proteins. Therefore, in this study, we use human CDB fused with both enhanced green fluorescent protein (EGFP) at C-terminus and red fluorescent protein (RFP, DsRed) at N-terminus in the transfected human melanoma cells to examine the effects of CDB on different fluorescent proteins. Our results indicated that CDB-fused fluorescent protein can be used to examine the slight gene regulations in the reporter gene system and have the potential to be the system for screening of functional compounds in the future. |
format | Online Article Text |
id | pubmed-3876668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38766682014-01-12 Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System Yang, Chao-Hsun Kuo, Wan-Ting Chuang, Yun-Ting Chen, Cheng-Yu Lin, Chih-Chien Biomed Res Int Research Article The periodic expression and destruction of several cyclins are the most important steps for the exact regulation of cell cycle. Cyclins are degraded by the ubiquitin-proteasome system during cell cycle. Besides, a short sequence near the N-terminal of cyclin B called the destruction box (D-box; CDB) is also required. Fluorescent-protein-based reporter gene system is insensitive to analysis because of the overly stable fluorescent proteins. Therefore, in this study, we use human CDB fused with both enhanced green fluorescent protein (EGFP) at C-terminus and red fluorescent protein (RFP, DsRed) at N-terminus in the transfected human melanoma cells to examine the effects of CDB on different fluorescent proteins. Our results indicated that CDB-fused fluorescent protein can be used to examine the slight gene regulations in the reporter gene system and have the potential to be the system for screening of functional compounds in the future. Hindawi Publishing Corporation 2013 2013-12-15 /pmc/articles/PMC3876668/ /pubmed/24416725 http://dx.doi.org/10.1155/2013/732307 Text en Copyright © 2013 Chao-Hsun Yang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yang, Chao-Hsun Kuo, Wan-Ting Chuang, Yun-Ting Chen, Cheng-Yu Lin, Chih-Chien Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System |
title | Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System |
title_full | Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System |
title_fullStr | Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System |
title_full_unstemmed | Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System |
title_short | Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System |
title_sort | cyclin b1 destruction box-mediated protein instability: the enhanced sensitivity of fluorescent-protein-based reporter gene system |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876668/ https://www.ncbi.nlm.nih.gov/pubmed/24416725 http://dx.doi.org/10.1155/2013/732307 |
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