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Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System

The periodic expression and destruction of several cyclins are the most important steps for the exact regulation of cell cycle. Cyclins are degraded by the ubiquitin-proteasome system during cell cycle. Besides, a short sequence near the N-terminal of cyclin B called the destruction box (D-box; CDB)...

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Autores principales: Yang, Chao-Hsun, Kuo, Wan-Ting, Chuang, Yun-Ting, Chen, Cheng-Yu, Lin, Chih-Chien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876668/
https://www.ncbi.nlm.nih.gov/pubmed/24416725
http://dx.doi.org/10.1155/2013/732307
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author Yang, Chao-Hsun
Kuo, Wan-Ting
Chuang, Yun-Ting
Chen, Cheng-Yu
Lin, Chih-Chien
author_facet Yang, Chao-Hsun
Kuo, Wan-Ting
Chuang, Yun-Ting
Chen, Cheng-Yu
Lin, Chih-Chien
author_sort Yang, Chao-Hsun
collection PubMed
description The periodic expression and destruction of several cyclins are the most important steps for the exact regulation of cell cycle. Cyclins are degraded by the ubiquitin-proteasome system during cell cycle. Besides, a short sequence near the N-terminal of cyclin B called the destruction box (D-box; CDB) is also required. Fluorescent-protein-based reporter gene system is insensitive to analysis because of the overly stable fluorescent proteins. Therefore, in this study, we use human CDB fused with both enhanced green fluorescent protein (EGFP) at C-terminus and red fluorescent protein (RFP, DsRed) at N-terminus in the transfected human melanoma cells to examine the effects of CDB on different fluorescent proteins. Our results indicated that CDB-fused fluorescent protein can be used to examine the slight gene regulations in the reporter gene system and have the potential to be the system for screening of functional compounds in the future.
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spelling pubmed-38766682014-01-12 Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System Yang, Chao-Hsun Kuo, Wan-Ting Chuang, Yun-Ting Chen, Cheng-Yu Lin, Chih-Chien Biomed Res Int Research Article The periodic expression and destruction of several cyclins are the most important steps for the exact regulation of cell cycle. Cyclins are degraded by the ubiquitin-proteasome system during cell cycle. Besides, a short sequence near the N-terminal of cyclin B called the destruction box (D-box; CDB) is also required. Fluorescent-protein-based reporter gene system is insensitive to analysis because of the overly stable fluorescent proteins. Therefore, in this study, we use human CDB fused with both enhanced green fluorescent protein (EGFP) at C-terminus and red fluorescent protein (RFP, DsRed) at N-terminus in the transfected human melanoma cells to examine the effects of CDB on different fluorescent proteins. Our results indicated that CDB-fused fluorescent protein can be used to examine the slight gene regulations in the reporter gene system and have the potential to be the system for screening of functional compounds in the future. Hindawi Publishing Corporation 2013 2013-12-15 /pmc/articles/PMC3876668/ /pubmed/24416725 http://dx.doi.org/10.1155/2013/732307 Text en Copyright © 2013 Chao-Hsun Yang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Chao-Hsun
Kuo, Wan-Ting
Chuang, Yun-Ting
Chen, Cheng-Yu
Lin, Chih-Chien
Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System
title Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System
title_full Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System
title_fullStr Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System
title_full_unstemmed Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System
title_short Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System
title_sort cyclin b1 destruction box-mediated protein instability: the enhanced sensitivity of fluorescent-protein-based reporter gene system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3876668/
https://www.ncbi.nlm.nih.gov/pubmed/24416725
http://dx.doi.org/10.1155/2013/732307
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