SNAP-Tag Technology Optimized for Use in Entamoeba histolytica

Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimi...

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Detalles Bibliográficos
Autores principales: Sateriale, Adam, Roy, Nathan H., Huston, Christopher D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877135/
https://www.ncbi.nlm.nih.gov/pubmed/24391864
http://dx.doi.org/10.1371/journal.pone.0083997
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author Sateriale, Adam
Roy, Nathan H.
Huston, Christopher D.
author_facet Sateriale, Adam
Roy, Nathan H.
Huston, Christopher D.
author_sort Sateriale, Adam
collection PubMed
description Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. We further demonstrate the capabilities of this system using super resolution microscopy, done for the first time in E. histolytica.
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spelling pubmed-38771352014-01-03 SNAP-Tag Technology Optimized for Use in Entamoeba histolytica Sateriale, Adam Roy, Nathan H. Huston, Christopher D. PLoS One Research Article Entamoeba histolytica is a protozoan parasite responsible for invasive intestinal and extraintestinal amebiasis. The pathology of amebiasis is still poorly understood, which can be largely attributed to lack of molecular tools. Here we present the optimization of SNAP-tag technology via codon optimization specific for E. histolytica. The resultant SNAP protein is highly expressed in amebic trophozoites, and shows proper localization when tagged with an endoplasmic reticulum retention signal. We further demonstrate the capabilities of this system using super resolution microscopy, done for the first time in E. histolytica. Public Library of Science 2013-12-31 /pmc/articles/PMC3877135/ /pubmed/24391864 http://dx.doi.org/10.1371/journal.pone.0083997 Text en © 2013 Sateriale et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sateriale, Adam
Roy, Nathan H.
Huston, Christopher D.
SNAP-Tag Technology Optimized for Use in Entamoeba histolytica
title SNAP-Tag Technology Optimized for Use in Entamoeba histolytica
title_full SNAP-Tag Technology Optimized for Use in Entamoeba histolytica
title_fullStr SNAP-Tag Technology Optimized for Use in Entamoeba histolytica
title_full_unstemmed SNAP-Tag Technology Optimized for Use in Entamoeba histolytica
title_short SNAP-Tag Technology Optimized for Use in Entamoeba histolytica
title_sort snap-tag technology optimized for use in entamoeba histolytica
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877135/
https://www.ncbi.nlm.nih.gov/pubmed/24391864
http://dx.doi.org/10.1371/journal.pone.0083997
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