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A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles
A gold nanoparticle (AuNP)-based colorimetric method was developed for the molecular weight (MW) determination of polyethylene glycol (PEG), a commonly used hydrophilic polymer. Addition of a salt solution to PEG-coated AuNP solutions helps in screening the electrostatic repulsion between nanopartic...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878192/ https://www.ncbi.nlm.nih.gov/pubmed/24359120 http://dx.doi.org/10.1186/1556-276X-8-538 |
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author | Ling, Kai Jiang, Hongyan Zhang, Qiqing |
author_facet | Ling, Kai Jiang, Hongyan Zhang, Qiqing |
author_sort | Ling, Kai |
collection | PubMed |
description | A gold nanoparticle (AuNP)-based colorimetric method was developed for the molecular weight (MW) determination of polyethylene glycol (PEG), a commonly used hydrophilic polymer. Addition of a salt solution to PEG-coated AuNP solutions helps in screening the electrostatic repulsion between nanoparticles and generating a color change of the solutions from wine red to blue in 10 min in accordance with the MW of PEG, which illustrates the different stability degrees (SDs) of the AuNPs. The SDs are calculated by the absorbance ratios of the stable to the aggregated AuNPs in the solution. The root mean square end-to-end length (〈h(2)〉(1/2)) of PEG molecules shows a linear fit to the SDs of the PEG-coated AuNPs in a range of 1.938 ± 0.156 to 10.151 ± 0.176 nm. According to the Derjaguin-Landau-Verwey-Overbeek theory, the reason for this linear relationship is that the thickness of the PEG adlayer is roughly equivalent to the 〈h(2)〉(1/2) of the PEG molecules in solution, which determines the SDs of the AuNPs. Subsequently, the MW of the PEG can be obtained from its 〈h(2)〉(1/2) using a mathematical relationship between 〈h(2)〉(1/2) and MW of PEG molecule. Applying this approach, we determined the 〈h(2)〉(1/2) and the MW of four PEG samples according to their absorbance values from the ordinary ultraviolet–visible spectrophotometric measurements. Therefore, the MW of PEG can be distinguished straightforwardly by visual inspection and determined by spectrophotometry. This novel approach is simple, rapid, and sensitive. |
format | Online Article Text |
id | pubmed-3878192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-38781922014-01-03 A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles Ling, Kai Jiang, Hongyan Zhang, Qiqing Nanoscale Res Lett Nano Express A gold nanoparticle (AuNP)-based colorimetric method was developed for the molecular weight (MW) determination of polyethylene glycol (PEG), a commonly used hydrophilic polymer. Addition of a salt solution to PEG-coated AuNP solutions helps in screening the electrostatic repulsion between nanoparticles and generating a color change of the solutions from wine red to blue in 10 min in accordance with the MW of PEG, which illustrates the different stability degrees (SDs) of the AuNPs. The SDs are calculated by the absorbance ratios of the stable to the aggregated AuNPs in the solution. The root mean square end-to-end length (〈h(2)〉(1/2)) of PEG molecules shows a linear fit to the SDs of the PEG-coated AuNPs in a range of 1.938 ± 0.156 to 10.151 ± 0.176 nm. According to the Derjaguin-Landau-Verwey-Overbeek theory, the reason for this linear relationship is that the thickness of the PEG adlayer is roughly equivalent to the 〈h(2)〉(1/2) of the PEG molecules in solution, which determines the SDs of the AuNPs. Subsequently, the MW of the PEG can be obtained from its 〈h(2)〉(1/2) using a mathematical relationship between 〈h(2)〉(1/2) and MW of PEG molecule. Applying this approach, we determined the 〈h(2)〉(1/2) and the MW of four PEG samples according to their absorbance values from the ordinary ultraviolet–visible spectrophotometric measurements. Therefore, the MW of PEG can be distinguished straightforwardly by visual inspection and determined by spectrophotometry. This novel approach is simple, rapid, and sensitive. Springer 2013-12-20 /pmc/articles/PMC3878192/ /pubmed/24359120 http://dx.doi.org/10.1186/1556-276X-8-538 Text en Copyright © 2013 Ling et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Nano Express Ling, Kai Jiang, Hongyan Zhang, Qiqing A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles |
title | A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles |
title_full | A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles |
title_fullStr | A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles |
title_full_unstemmed | A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles |
title_short | A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles |
title_sort | colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878192/ https://www.ncbi.nlm.nih.gov/pubmed/24359120 http://dx.doi.org/10.1186/1556-276X-8-538 |
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