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Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function
BACKGROUND: The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effo...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC387831/ https://www.ncbi.nlm.nih.gov/pubmed/15102337 http://dx.doi.org/10.1186/1472-6807-4-5 |
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author | Teplyakov, Alexey Pullalarevu, Sadhana Obmolova, Galina Doseeva, Victoria Galkin, Andrey Herzberg, Osnat Dauter, Miroslawa Dauter, Zbigniew Gilliland, Gary L |
author_facet | Teplyakov, Alexey Pullalarevu, Sadhana Obmolova, Galina Doseeva, Victoria Galkin, Andrey Herzberg, Osnat Dauter, Miroslawa Dauter, Zbigniew Gilliland, Gary L |
author_sort | Teplyakov, Alexey |
collection | PubMed |
description | BACKGROUND: The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. RESULTS: The structure was determined at 1.0 Å resolution by single-wavelength anomalous diffraction. The fold is very similar to that of arsenate reductase, which is an extension of the thioredoxin fold. CONCLUSION: Given the conservation of the functionally important residues and the ability to bind glutathione, YffB is likely to function as a GSH-dependent thiol reductase. |
format | Text |
id | pubmed-387831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-3878312004-04-17 Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function Teplyakov, Alexey Pullalarevu, Sadhana Obmolova, Galina Doseeva, Victoria Galkin, Andrey Herzberg, Osnat Dauter, Miroslawa Dauter, Zbigniew Gilliland, Gary L BMC Struct Biol Research Article BACKGROUND: The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. RESULTS: The structure was determined at 1.0 Å resolution by single-wavelength anomalous diffraction. The fold is very similar to that of arsenate reductase, which is an extension of the thioredoxin fold. CONCLUSION: Given the conservation of the functionally important residues and the ability to bind glutathione, YffB is likely to function as a GSH-dependent thiol reductase. BioMed Central 2004-03-08 /pmc/articles/PMC387831/ /pubmed/15102337 http://dx.doi.org/10.1186/1472-6807-4-5 Text en Copyright © 2004 Teplyakov et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Article Teplyakov, Alexey Pullalarevu, Sadhana Obmolova, Galina Doseeva, Victoria Galkin, Andrey Herzberg, Osnat Dauter, Miroslawa Dauter, Zbigniew Gilliland, Gary L Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function |
title | Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function |
title_full | Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function |
title_fullStr | Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function |
title_full_unstemmed | Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function |
title_short | Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function |
title_sort | crystal structure of the yffb protein from pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC387831/ https://www.ncbi.nlm.nih.gov/pubmed/15102337 http://dx.doi.org/10.1186/1472-6807-4-5 |
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