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Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function

BACKGROUND: The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effo...

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Autores principales: Teplyakov, Alexey, Pullalarevu, Sadhana, Obmolova, Galina, Doseeva, Victoria, Galkin, Andrey, Herzberg, Osnat, Dauter, Miroslawa, Dauter, Zbigniew, Gilliland, Gary L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC387831/
https://www.ncbi.nlm.nih.gov/pubmed/15102337
http://dx.doi.org/10.1186/1472-6807-4-5
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author Teplyakov, Alexey
Pullalarevu, Sadhana
Obmolova, Galina
Doseeva, Victoria
Galkin, Andrey
Herzberg, Osnat
Dauter, Miroslawa
Dauter, Zbigniew
Gilliland, Gary L
author_facet Teplyakov, Alexey
Pullalarevu, Sadhana
Obmolova, Galina
Doseeva, Victoria
Galkin, Andrey
Herzberg, Osnat
Dauter, Miroslawa
Dauter, Zbigniew
Gilliland, Gary L
author_sort Teplyakov, Alexey
collection PubMed
description BACKGROUND: The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. RESULTS: The structure was determined at 1.0 Å resolution by single-wavelength anomalous diffraction. The fold is very similar to that of arsenate reductase, which is an extension of the thioredoxin fold. CONCLUSION: Given the conservation of the functionally important residues and the ability to bind glutathione, YffB is likely to function as a GSH-dependent thiol reductase.
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spelling pubmed-3878312004-04-17 Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function Teplyakov, Alexey Pullalarevu, Sadhana Obmolova, Galina Doseeva, Victoria Galkin, Andrey Herzberg, Osnat Dauter, Miroslawa Dauter, Zbigniew Gilliland, Gary L BMC Struct Biol Research Article BACKGROUND: The yffB (PA3664) gene of Pseudomonas aeruginosa encodes an uncharacterized protein of 13 kDa molecular weight with a marginal sequence similarity to arsenate reductase from Escherichia coli. The crystal structure determination of YffB was undertaken as part of a structural genomics effort in order to assist with the functional assignment of the protein. RESULTS: The structure was determined at 1.0 Å resolution by single-wavelength anomalous diffraction. The fold is very similar to that of arsenate reductase, which is an extension of the thioredoxin fold. CONCLUSION: Given the conservation of the functionally important residues and the ability to bind glutathione, YffB is likely to function as a GSH-dependent thiol reductase. BioMed Central 2004-03-08 /pmc/articles/PMC387831/ /pubmed/15102337 http://dx.doi.org/10.1186/1472-6807-4-5 Text en Copyright © 2004 Teplyakov et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Teplyakov, Alexey
Pullalarevu, Sadhana
Obmolova, Galina
Doseeva, Victoria
Galkin, Andrey
Herzberg, Osnat
Dauter, Miroslawa
Dauter, Zbigniew
Gilliland, Gary L
Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function
title Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function
title_full Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function
title_fullStr Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function
title_full_unstemmed Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function
title_short Crystal structure of the YffB protein from Pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function
title_sort crystal structure of the yffb protein from pseudomonas aeruginosa suggests a glutathione-dependent thiol reductase function
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC387831/
https://www.ncbi.nlm.nih.gov/pubmed/15102337
http://dx.doi.org/10.1186/1472-6807-4-5
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