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Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns

BACKGROUND: Escherichia coli O157 (E. coli O157) has been isolated from bison retail meat, a fact that is important given that bison meat has been implicated in an E. coli O157-multistate outbreak. In addition, E. coli O157 has also been isolated from bison feces at slaughter and on farms. Cattle ar...

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Autores principales: Kudva, Indira T, Stasko, Judith A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878412/
https://www.ncbi.nlm.nih.gov/pubmed/24373611
http://dx.doi.org/10.1186/1746-6148-9-266
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author Kudva, Indira T
Stasko, Judith A
author_facet Kudva, Indira T
Stasko, Judith A
author_sort Kudva, Indira T
collection PubMed
description BACKGROUND: Escherichia coli O157 (E. coli O157) has been isolated from bison retail meat, a fact that is important given that bison meat has been implicated in an E. coli O157-multistate outbreak. In addition, E. coli O157 has also been isolated from bison feces at slaughter and on farms. Cattle are well documented as E. coli O157 reservoirs, and the primary site of E. coli O157 persistence in such reservoirs is the rectoanal junction (RAJ), located at the distal end of the bovine gastrointestinal tract. Since bison and cattle share many genetic similarities manifested as common lineage, susceptibility to infection and the nature of immune responses to infectious agents, we decided to evaluate whether the RAJ of these animals were comparable both in terms of cellular architecture and as sites for adherence of E. coli O157. Specifically, we compared the histo-morphologies of the RAJ and evaluated the E. coli O157 adherence characteristics to the RAJ squamous epithelial (RSE) cells, from these two species. RESULTS: We found that the RAJ of both bison and cattle demonstrated similar distribution of epithelial cell markers villin, vimentin, cytokeratin, E-cadherin and N-cadherin. Interestingly, N-cadherin predominated in the stratified squamous epithelium reflecting its proliferative nature. E. coli O157 strains 86–24 Sm(R) and EDL 933 adhered to RSE cells from both animals with similar diffuse and aggregative patterns, respectively. CONCLUSION: Our observations further support the fact that bison are likely ‘wildlife’ reservoirs for E. coli O157, harboring these bacteria in their gastrointestinal tract. Our results also extend the utility of the RSE-cell assay, previously developed to elucidate E. coli O157-cattle RAJ interactions, to studies in bison, which are warranted to determine whether these observations in vitro correlate with those occurring in vivo at the RAJ within the bison gastrointestinal tract.
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spelling pubmed-38784122014-01-03 Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns Kudva, Indira T Stasko, Judith A BMC Vet Res Research Article BACKGROUND: Escherichia coli O157 (E. coli O157) has been isolated from bison retail meat, a fact that is important given that bison meat has been implicated in an E. coli O157-multistate outbreak. In addition, E. coli O157 has also been isolated from bison feces at slaughter and on farms. Cattle are well documented as E. coli O157 reservoirs, and the primary site of E. coli O157 persistence in such reservoirs is the rectoanal junction (RAJ), located at the distal end of the bovine gastrointestinal tract. Since bison and cattle share many genetic similarities manifested as common lineage, susceptibility to infection and the nature of immune responses to infectious agents, we decided to evaluate whether the RAJ of these animals were comparable both in terms of cellular architecture and as sites for adherence of E. coli O157. Specifically, we compared the histo-morphologies of the RAJ and evaluated the E. coli O157 adherence characteristics to the RAJ squamous epithelial (RSE) cells, from these two species. RESULTS: We found that the RAJ of both bison and cattle demonstrated similar distribution of epithelial cell markers villin, vimentin, cytokeratin, E-cadherin and N-cadherin. Interestingly, N-cadherin predominated in the stratified squamous epithelium reflecting its proliferative nature. E. coli O157 strains 86–24 Sm(R) and EDL 933 adhered to RSE cells from both animals with similar diffuse and aggregative patterns, respectively. CONCLUSION: Our observations further support the fact that bison are likely ‘wildlife’ reservoirs for E. coli O157, harboring these bacteria in their gastrointestinal tract. Our results also extend the utility of the RSE-cell assay, previously developed to elucidate E. coli O157-cattle RAJ interactions, to studies in bison, which are warranted to determine whether these observations in vitro correlate with those occurring in vivo at the RAJ within the bison gastrointestinal tract. BioMed Central 2013-12-28 /pmc/articles/PMC3878412/ /pubmed/24373611 http://dx.doi.org/10.1186/1746-6148-9-266 Text en Copyright © 2013 Kudva and Stasko; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kudva, Indira T
Stasko, Judith A
Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns
title Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns
title_full Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns
title_fullStr Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns
title_full_unstemmed Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns
title_short Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns
title_sort bison and bovine rectoanal junctions exhibit similar cellular architecture and escherichia coli o157 adherence patterns
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878412/
https://www.ncbi.nlm.nih.gov/pubmed/24373611
http://dx.doi.org/10.1186/1746-6148-9-266
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