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Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants
BACKGROUND: Wheat and rice are important food crops with enormous biomass residues for biofuels. However, lignocellulosic recalcitrance becomes a crucial factor on biomass process. Plant cell walls greatly determine biomass recalcitrance, thus it is essential to identify their key factors on lignoce...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878626/ https://www.ncbi.nlm.nih.gov/pubmed/24341349 http://dx.doi.org/10.1186/1754-6834-6-183 |
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author | Wu, Zhiliang Zhang, Mingliang Wang, Lingqiang Tu, Yuanyuan Zhang, Jing Xie, Guosheng Zou, Weihua Li, Fengcheng Guo, Kai Li, Qing Gao, Chunbao Peng, Liangcai |
author_facet | Wu, Zhiliang Zhang, Mingliang Wang, Lingqiang Tu, Yuanyuan Zhang, Jing Xie, Guosheng Zou, Weihua Li, Fengcheng Guo, Kai Li, Qing Gao, Chunbao Peng, Liangcai |
author_sort | Wu, Zhiliang |
collection | PubMed |
description | BACKGROUND: Wheat and rice are important food crops with enormous biomass residues for biofuels. However, lignocellulosic recalcitrance becomes a crucial factor on biomass process. Plant cell walls greatly determine biomass recalcitrance, thus it is essential to identify their key factors on lignocellulose saccharification. Despite it has been reported about cell wall factors on biomass digestions, little is known in wheat and rice. In this study, we analyzed nine typical pairs of wheat and rice samples that exhibited distinct cell wall compositions, and identified three major factors of wall polymer features that affected biomass digestibility. RESULTS: Based on cell wall compositions, ten wheat accessions and three rice mutants were classified into three distinct groups each with three typical pairs. In terms of group I that displayed single wall polymer alternations in wheat, we found that three wall polymer levels (cellulose, hemicelluloses and lignin) each had a negative effect on biomass digestibility at similar rates under pretreatments of NaOH and H(2)SO(4) with three concentrations. However, analysis of six pairs of wheat and rice samples in groups II and III that each exhibited a similar cell wall composition, indicated that three wall polymer levels were not the major factors on biomass saccharification. Furthermore, in-depth detection of the wall polymer features distinctive in rice mutants, demonstrated that biomass digestibility was remarkably affected either negatively by cellulose crystallinity (CrI) of raw biomass materials, or positively by both Ara substitution degree of non-KOH-extractable hemicelluloses (reverse Xyl/Ara) and p-coumaryl alcohol relative proportion of KOH-extractable lignin (H/G). Correlation analysis indicated that Ara substitution degree and H/G ratio negatively affected cellulose crystallinity for high biomass enzymatic digestion. It was also suggested to determine whether Ara and H monomer have an interlinking with cellulose chains in the future. CONCLUSIONS: Using nine typical pairs of wheat and rice samples having distinct cell wall compositions and wide biomass saccharification, Ara substitution degree and monolignin H proportion have been revealed to be the dominant factors positively determining biomass digestibility upon various chemical pretreatments. The results demonstrated the potential of genetic modification of plant cell walls for high biomass saccharification in bioenergy crops. |
format | Online Article Text |
id | pubmed-3878626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-38786262014-01-07 Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants Wu, Zhiliang Zhang, Mingliang Wang, Lingqiang Tu, Yuanyuan Zhang, Jing Xie, Guosheng Zou, Weihua Li, Fengcheng Guo, Kai Li, Qing Gao, Chunbao Peng, Liangcai Biotechnol Biofuels Research BACKGROUND: Wheat and rice are important food crops with enormous biomass residues for biofuels. However, lignocellulosic recalcitrance becomes a crucial factor on biomass process. Plant cell walls greatly determine biomass recalcitrance, thus it is essential to identify their key factors on lignocellulose saccharification. Despite it has been reported about cell wall factors on biomass digestions, little is known in wheat and rice. In this study, we analyzed nine typical pairs of wheat and rice samples that exhibited distinct cell wall compositions, and identified three major factors of wall polymer features that affected biomass digestibility. RESULTS: Based on cell wall compositions, ten wheat accessions and three rice mutants were classified into three distinct groups each with three typical pairs. In terms of group I that displayed single wall polymer alternations in wheat, we found that three wall polymer levels (cellulose, hemicelluloses and lignin) each had a negative effect on biomass digestibility at similar rates under pretreatments of NaOH and H(2)SO(4) with three concentrations. However, analysis of six pairs of wheat and rice samples in groups II and III that each exhibited a similar cell wall composition, indicated that three wall polymer levels were not the major factors on biomass saccharification. Furthermore, in-depth detection of the wall polymer features distinctive in rice mutants, demonstrated that biomass digestibility was remarkably affected either negatively by cellulose crystallinity (CrI) of raw biomass materials, or positively by both Ara substitution degree of non-KOH-extractable hemicelluloses (reverse Xyl/Ara) and p-coumaryl alcohol relative proportion of KOH-extractable lignin (H/G). Correlation analysis indicated that Ara substitution degree and H/G ratio negatively affected cellulose crystallinity for high biomass enzymatic digestion. It was also suggested to determine whether Ara and H monomer have an interlinking with cellulose chains in the future. CONCLUSIONS: Using nine typical pairs of wheat and rice samples having distinct cell wall compositions and wide biomass saccharification, Ara substitution degree and monolignin H proportion have been revealed to be the dominant factors positively determining biomass digestibility upon various chemical pretreatments. The results demonstrated the potential of genetic modification of plant cell walls for high biomass saccharification in bioenergy crops. BioMed Central 2013-12-16 /pmc/articles/PMC3878626/ /pubmed/24341349 http://dx.doi.org/10.1186/1754-6834-6-183 Text en Copyright © 2013 Wu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Wu, Zhiliang Zhang, Mingliang Wang, Lingqiang Tu, Yuanyuan Zhang, Jing Xie, Guosheng Zou, Weihua Li, Fengcheng Guo, Kai Li, Qing Gao, Chunbao Peng, Liangcai Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants |
title | Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants |
title_full | Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants |
title_fullStr | Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants |
title_full_unstemmed | Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants |
title_short | Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants |
title_sort | biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878626/ https://www.ncbi.nlm.nih.gov/pubmed/24341349 http://dx.doi.org/10.1186/1754-6834-6-183 |
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