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Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC

BACKGROUND: Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage w...

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Autores principales: McGaw, Lyndy Joy, Bagla, Victor Patrick, Steenkamp, Paul Anton, Fouche, Gerda, Olivier, Jana, Eloff, Jacobus Nicolaas, Myer, Martin Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878802/
https://www.ncbi.nlm.nih.gov/pubmed/24330447
http://dx.doi.org/10.1186/1472-6882-13-356
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author McGaw, Lyndy Joy
Bagla, Victor Patrick
Steenkamp, Paul Anton
Fouche, Gerda
Olivier, Jana
Eloff, Jacobus Nicolaas
Myer, Martin Steven
author_facet McGaw, Lyndy Joy
Bagla, Victor Patrick
Steenkamp, Paul Anton
Fouche, Gerda
Olivier, Jana
Eloff, Jacobus Nicolaas
Myer, Martin Steven
author_sort McGaw, Lyndy Joy
collection PubMed
description BACKGROUND: Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commercialization potential. METHODS: Extracts of the dried ground aerial parts were prepared using organic solvents (diethyl ether, dichloromethane/methanol, ethyl acetate and ethanol) and water. These extracts were subjected to HPLC, TLC and bioautography analysis with the aim of linking a range of peaks visualized in HPLC chromatography profiles to antibacterial and antifungal activity of the same extracts. RESULTS: HPLC revealed a group of compounds extracted by more than one solvent. Compounds identified include inositol, caffeic acid, quercetin, kaempferol, apigenin, hymenoxin and oleanolic acid. The organic extracts displayed similar TLC profiles, and bioautography indicated approximately five antibacterial compounds, but only two antifungal compounds in these extracts. Bioautography indicated that cold water extracted the least antimicrobial compounds. CONCLUSIONS: Several previously unknown compounds were identified in Athrixia phylicoides extracts, and bioautography indicated a number of antibacterial and antifungal compounds. There were notable differences in chemical composition and bioactivity between the organic and aqueous extracts. Further research is necessary to fully characterize the active components of the extracts.
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spelling pubmed-38788022014-01-03 Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC McGaw, Lyndy Joy Bagla, Victor Patrick Steenkamp, Paul Anton Fouche, Gerda Olivier, Jana Eloff, Jacobus Nicolaas Myer, Martin Steven BMC Complement Altern Med Research Article BACKGROUND: Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commercialization potential. METHODS: Extracts of the dried ground aerial parts were prepared using organic solvents (diethyl ether, dichloromethane/methanol, ethyl acetate and ethanol) and water. These extracts were subjected to HPLC, TLC and bioautography analysis with the aim of linking a range of peaks visualized in HPLC chromatography profiles to antibacterial and antifungal activity of the same extracts. RESULTS: HPLC revealed a group of compounds extracted by more than one solvent. Compounds identified include inositol, caffeic acid, quercetin, kaempferol, apigenin, hymenoxin and oleanolic acid. The organic extracts displayed similar TLC profiles, and bioautography indicated approximately five antibacterial compounds, but only two antifungal compounds in these extracts. Bioautography indicated that cold water extracted the least antimicrobial compounds. CONCLUSIONS: Several previously unknown compounds were identified in Athrixia phylicoides extracts, and bioautography indicated a number of antibacterial and antifungal compounds. There were notable differences in chemical composition and bioactivity between the organic and aqueous extracts. Further research is necessary to fully characterize the active components of the extracts. BioMed Central 2013-12-13 /pmc/articles/PMC3878802/ /pubmed/24330447 http://dx.doi.org/10.1186/1472-6882-13-356 Text en Copyright © 2013 McGaw et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
McGaw, Lyndy Joy
Bagla, Victor Patrick
Steenkamp, Paul Anton
Fouche, Gerda
Olivier, Jana
Eloff, Jacobus Nicolaas
Myer, Martin Steven
Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC
title Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC
title_full Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC
title_fullStr Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC
title_full_unstemmed Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC
title_short Antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of Athrixia phylicoides determined using bioautography and HPLC
title_sort antifungal and antibacterial activity and chemical composition of polar and non-polar extracts of athrixia phylicoides determined using bioautography and hplc
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878802/
https://www.ncbi.nlm.nih.gov/pubmed/24330447
http://dx.doi.org/10.1186/1472-6882-13-356
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