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Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection

Infections in cattle with the gastric nematode Ostertagia ostertagi are associated with decreased acid secretion and profound physio-morphological changes of the gastric mucosa. The purpose of the current study was to investigate the mechanisms triggering these pathophysiological changes. O. osterta...

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Autores principales: Mihi, Belgacem, Van Meulder, Frederik, Rinaldi, Manuela, Van Coppernolle, Stefanie, Chiers, Koen, Van den Broeck, Wim, Goddeeris, Bruno, Vercruysse, Jozef, Claerebout, Edwin, Geldhof, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878833/
https://www.ncbi.nlm.nih.gov/pubmed/24330735
http://dx.doi.org/10.1186/1297-9716-44-121
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author Mihi, Belgacem
Van Meulder, Frederik
Rinaldi, Manuela
Van Coppernolle, Stefanie
Chiers, Koen
Van den Broeck, Wim
Goddeeris, Bruno
Vercruysse, Jozef
Claerebout, Edwin
Geldhof, Peter
author_facet Mihi, Belgacem
Van Meulder, Frederik
Rinaldi, Manuela
Van Coppernolle, Stefanie
Chiers, Koen
Van den Broeck, Wim
Goddeeris, Bruno
Vercruysse, Jozef
Claerebout, Edwin
Geldhof, Peter
author_sort Mihi, Belgacem
collection PubMed
description Infections in cattle with the gastric nematode Ostertagia ostertagi are associated with decreased acid secretion and profound physio-morphological changes of the gastric mucosa. The purpose of the current study was to investigate the mechanisms triggering these pathophysiological changes. O. ostertagi infection resulted in a marked cellular hyperplasia, which can be explained by increased transcriptional levels of signaling molecules related to the homeostasis of gastric epithelial cells such as HES1, WNT5A, FGF10, HB-EGF, AREG, ADAM10 and ADAM17. Intriguingly, histological analysis indicated that the rapid rise in the gastric pH, observed following the emergence of adult worms, cannot be explained by a loss of parietal cells, as a decrease in the number of parietal cells was only observed following a long term infection of several weeks, but is likely to be caused by an inhibition of parietal cell activity. To investigate whether this inhibition is caused by a direct effect of the parasites, parietal cells were co-cultured with parasite Excretory/Secretory products (ESP) and subsequently analyzed for acid production. The results indicate that adult ESP inhibited acid secretion, whereas ESP from the L4 larval stages did not alter parietal cell function. In addition, our data show that the inhibition of parietal cell activity could be mediated by a marked upregulation of inflammatory factors, which are partly induced by adult ESP in abomasal epithelial cells. In conclusion, this study shows that the emergence of adult O. ostertagi worms is associated with marked cellular changes that can be partly triggered by the worm’s Excretory/secretory antigens.
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spelling pubmed-38788332014-01-03 Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection Mihi, Belgacem Van Meulder, Frederik Rinaldi, Manuela Van Coppernolle, Stefanie Chiers, Koen Van den Broeck, Wim Goddeeris, Bruno Vercruysse, Jozef Claerebout, Edwin Geldhof, Peter Vet Res Research Infections in cattle with the gastric nematode Ostertagia ostertagi are associated with decreased acid secretion and profound physio-morphological changes of the gastric mucosa. The purpose of the current study was to investigate the mechanisms triggering these pathophysiological changes. O. ostertagi infection resulted in a marked cellular hyperplasia, which can be explained by increased transcriptional levels of signaling molecules related to the homeostasis of gastric epithelial cells such as HES1, WNT5A, FGF10, HB-EGF, AREG, ADAM10 and ADAM17. Intriguingly, histological analysis indicated that the rapid rise in the gastric pH, observed following the emergence of adult worms, cannot be explained by a loss of parietal cells, as a decrease in the number of parietal cells was only observed following a long term infection of several weeks, but is likely to be caused by an inhibition of parietal cell activity. To investigate whether this inhibition is caused by a direct effect of the parasites, parietal cells were co-cultured with parasite Excretory/Secretory products (ESP) and subsequently analyzed for acid production. The results indicate that adult ESP inhibited acid secretion, whereas ESP from the L4 larval stages did not alter parietal cell function. In addition, our data show that the inhibition of parietal cell activity could be mediated by a marked upregulation of inflammatory factors, which are partly induced by adult ESP in abomasal epithelial cells. In conclusion, this study shows that the emergence of adult O. ostertagi worms is associated with marked cellular changes that can be partly triggered by the worm’s Excretory/secretory antigens. BioMed Central 2013 2013-12-11 /pmc/articles/PMC3878833/ /pubmed/24330735 http://dx.doi.org/10.1186/1297-9716-44-121 Text en Copyright © 2013 Mihi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mihi, Belgacem
Van Meulder, Frederik
Rinaldi, Manuela
Van Coppernolle, Stefanie
Chiers, Koen
Van den Broeck, Wim
Goddeeris, Bruno
Vercruysse, Jozef
Claerebout, Edwin
Geldhof, Peter
Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection
title Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection
title_full Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection
title_fullStr Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection
title_full_unstemmed Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection
title_short Analysis of cell hyperplasia and parietal cell dysfunction induced by Ostertagia ostertagi infection
title_sort analysis of cell hyperplasia and parietal cell dysfunction induced by ostertagia ostertagi infection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3878833/
https://www.ncbi.nlm.nih.gov/pubmed/24330735
http://dx.doi.org/10.1186/1297-9716-44-121
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