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Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome

OBJECTIVE: Irritable bowel syndrome (IBS) is a common clinical gastrointestinal dysfunction disorders. 5-sertonon (5-hydroxytryptamine, 5-HT) is a very important neurotransmitter, which is involved in gastrointestinal motion and sensation. Solute carrier family 6 member 4 (SLC6A4) gene encode seroto...

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Autores principales: Yuan, Jing, Kang, ChuanYuan, Wang, Min, Wang, Qiang, Li, PeiKai, Liu, Hua, Hou, Yu, Su, Ping, Yang, Fan, Wei, YuJun, Yang, JianZhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879322/
https://www.ncbi.nlm.nih.gov/pubmed/24392134
http://dx.doi.org/10.1371/journal.pone.0084414
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author Yuan, Jing
Kang, ChuanYuan
Wang, Min
Wang, Qiang
Li, PeiKai
Liu, Hua
Hou, Yu
Su, Ping
Yang, Fan
Wei, YuJun
Yang, JianZhong
author_facet Yuan, Jing
Kang, ChuanYuan
Wang, Min
Wang, Qiang
Li, PeiKai
Liu, Hua
Hou, Yu
Su, Ping
Yang, Fan
Wei, YuJun
Yang, JianZhong
author_sort Yuan, Jing
collection PubMed
description OBJECTIVE: Irritable bowel syndrome (IBS) is a common clinical gastrointestinal dysfunction disorders. 5-sertonon (5-hydroxytryptamine, 5-HT) is a very important neurotransmitter, which is involved in gastrointestinal motion and sensation. Solute carrier family 6 member 4 (SLC6A4) gene encode serotonin transporter (SERT) which function is to rapidly reuptake the most of 5-HT. Therefore, it is needed to explore the association between SLC6A4 gene polymorphisms and IBS. METHODS: 119 patients and 238 healthy controls were administrated to detect the SLC6A4 gene polymorphisms including 5-HT-transporter-gene-linked polymorphic region (5-HTTLPR), variable number of tandem repeats (VNTRs) and three selected tag Single Nucleotide Polymorphisms (SNPs) rs1042173, rs3794808, rs2020936 by using polymerase chain reaction (PCR) and TaqMan® SNP Genotyping. RESULTS: There were significant difference for 5-HTTLPR between IBS and control groups (X2 = 106.168, P<0.0001). In control group, genotypes were mainly L/L (58.4%), however, the genotypes in IBS were S/S (37.8%). The significant difference was shown in D-IBS subjects when compared to the controls (X(2) = 50.850, P<0.0001) for 5-HTTLPR. For STin2 VNTR, rs1042173, rs3794808, and rs2020936 polymorphisms, there were no any significant differences between IBS and control groups. There were no statistical significantly haplotypes for 5-HTTLPR, VNTRs and the three SNPs between IBS and controls. CONCLUSION: The S allele in 5-HTTLPR was a susceptible allele with Chinese Han IBS, but other associations of VNTRs, three selected Tag SNPs and positive haplotype with IBS were not found. It is indicated that much research are needed to study the relationship between other polymorphisms in SLC6A4 gene and IBS.
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spelling pubmed-38793222014-01-03 Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome Yuan, Jing Kang, ChuanYuan Wang, Min Wang, Qiang Li, PeiKai Liu, Hua Hou, Yu Su, Ping Yang, Fan Wei, YuJun Yang, JianZhong PLoS One Research Article OBJECTIVE: Irritable bowel syndrome (IBS) is a common clinical gastrointestinal dysfunction disorders. 5-sertonon (5-hydroxytryptamine, 5-HT) is a very important neurotransmitter, which is involved in gastrointestinal motion and sensation. Solute carrier family 6 member 4 (SLC6A4) gene encode serotonin transporter (SERT) which function is to rapidly reuptake the most of 5-HT. Therefore, it is needed to explore the association between SLC6A4 gene polymorphisms and IBS. METHODS: 119 patients and 238 healthy controls were administrated to detect the SLC6A4 gene polymorphisms including 5-HT-transporter-gene-linked polymorphic region (5-HTTLPR), variable number of tandem repeats (VNTRs) and three selected tag Single Nucleotide Polymorphisms (SNPs) rs1042173, rs3794808, rs2020936 by using polymerase chain reaction (PCR) and TaqMan® SNP Genotyping. RESULTS: There were significant difference for 5-HTTLPR between IBS and control groups (X2 = 106.168, P<0.0001). In control group, genotypes were mainly L/L (58.4%), however, the genotypes in IBS were S/S (37.8%). The significant difference was shown in D-IBS subjects when compared to the controls (X(2) = 50.850, P<0.0001) for 5-HTTLPR. For STin2 VNTR, rs1042173, rs3794808, and rs2020936 polymorphisms, there were no any significant differences between IBS and control groups. There were no statistical significantly haplotypes for 5-HTTLPR, VNTRs and the three SNPs between IBS and controls. CONCLUSION: The S allele in 5-HTTLPR was a susceptible allele with Chinese Han IBS, but other associations of VNTRs, three selected Tag SNPs and positive haplotype with IBS were not found. It is indicated that much research are needed to study the relationship between other polymorphisms in SLC6A4 gene and IBS. Public Library of Science 2014-01-02 /pmc/articles/PMC3879322/ /pubmed/24392134 http://dx.doi.org/10.1371/journal.pone.0084414 Text en © 2014 Yuan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yuan, Jing
Kang, ChuanYuan
Wang, Min
Wang, Qiang
Li, PeiKai
Liu, Hua
Hou, Yu
Su, Ping
Yang, Fan
Wei, YuJun
Yang, JianZhong
Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome
title Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome
title_full Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome
title_fullStr Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome
title_full_unstemmed Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome
title_short Association Study of Serotonin Transporter SLC6A4 Gene with Chinese Han Irritable Bowel Syndrome
title_sort association study of serotonin transporter slc6a4 gene with chinese han irritable bowel syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879322/
https://www.ncbi.nlm.nih.gov/pubmed/24392134
http://dx.doi.org/10.1371/journal.pone.0084414
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