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An Improved Protocol for Intact Chloroplasts and cpDNA Isolation in Conifers

BACKGROUND: Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid genome sequencing pro...

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Detalles Bibliográficos
Autores principales:	Vieira, Leila do Nascimento, Faoro, Helisson, Fraga, Hugo Pacheco de Freitas, Rogalski, Marcelo, de Souza, Emanuel Maltempi, de Oliveira Pedrosa, Fábio, Nodari, Rubens Onofre, Guerra, Miguel Pedro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879346/ https://www.ncbi.nlm.nih.gov/pubmed/24392157 http://dx.doi.org/10.1371/journal.pone.0084792

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