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Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species

BACKGROUND: Jatropha curcas L. is an oil seed producing non-leguminous tropical shrub that has good potential to be a fuel plant that can be cultivated on marginal land. Due to the low nutrient content of the targeted plantation area, the requirement for fertilizer is expected to be higher than othe...

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Autores principales: Madhaiyan, Munusamy, Peng, Ni, Te, Ngoh Si, Hsin I, Cheng, Lin, Cai, Lin, Fu, Reddy, Chalapathy, Yan, Hong, Ji, Lianghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879406/
https://www.ncbi.nlm.nih.gov/pubmed/24083555
http://dx.doi.org/10.1186/1754-6834-6-140
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author Madhaiyan, Munusamy
Peng, Ni
Te, Ngoh Si
Hsin I, Cheng
Lin, Cai
Lin, Fu
Reddy, Chalapathy
Yan, Hong
Ji, Lianghui
author_facet Madhaiyan, Munusamy
Peng, Ni
Te, Ngoh Si
Hsin I, Cheng
Lin, Cai
Lin, Fu
Reddy, Chalapathy
Yan, Hong
Ji, Lianghui
author_sort Madhaiyan, Munusamy
collection PubMed
description BACKGROUND: Jatropha curcas L. is an oil seed producing non-leguminous tropical shrub that has good potential to be a fuel plant that can be cultivated on marginal land. Due to the low nutrient content of the targeted plantation area, the requirement for fertilizer is expected to be higher than other plants. This factor severely affects the commercial viability of J. curcas. RESULTS: We explored the feasibility to use endophytic nitrogen-fixing bacteria that are native to J. curcas to improve plant growth, biomass and seed productivity. We demonstrated that a novel N-fixing endophyte, Enterobacter sp. R4-368, was able to colonize in root and stem tissues and significantly promoted early plant growth and seed productivity of J. curcas in sterilized and non-sterilized soil. Inoculation of young seedling led to an approximately 57.2% increase in seedling vigour over a six week period. At 90 days after planting, inoculated plants showed an average increase of 25.3%, 77.7%, 27.5%, 45.8% in plant height, leaf number, chlorophyll content and stem volume, respectively. Notably, inoculation of the strain led to a 49.0% increase in the average seed number per plant and 20% increase in the average single seed weight when plants were maintained for 1.5 years in non-sterilized soil in pots in the open air. Enterobacter sp. R4-368 cells were able to colonize root tissues and moved systemically to stem tissues. However, no bacteria were found in leaves. Promotion of plant growth and leaf nitrogen content by the strain was partially lost in nifH, nifD, nifK knockout mutants, suggesting the presence of other growth promoting factors that are associated with this bacterium strain. CONCLUSION: Our results showed that Enterobacter sp. R4-368 significantly promoted growth and seed yield of J. curcas. The application of the strains is likely to significantly improve the commercial viability of J. curcas due to the reduced fertilizer cost and improved oil yield.
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spelling pubmed-38794062014-01-04 Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species Madhaiyan, Munusamy Peng, Ni Te, Ngoh Si Hsin I, Cheng Lin, Cai Lin, Fu Reddy, Chalapathy Yan, Hong Ji, Lianghui Biotechnol Biofuels Research BACKGROUND: Jatropha curcas L. is an oil seed producing non-leguminous tropical shrub that has good potential to be a fuel plant that can be cultivated on marginal land. Due to the low nutrient content of the targeted plantation area, the requirement for fertilizer is expected to be higher than other plants. This factor severely affects the commercial viability of J. curcas. RESULTS: We explored the feasibility to use endophytic nitrogen-fixing bacteria that are native to J. curcas to improve plant growth, biomass and seed productivity. We demonstrated that a novel N-fixing endophyte, Enterobacter sp. R4-368, was able to colonize in root and stem tissues and significantly promoted early plant growth and seed productivity of J. curcas in sterilized and non-sterilized soil. Inoculation of young seedling led to an approximately 57.2% increase in seedling vigour over a six week period. At 90 days after planting, inoculated plants showed an average increase of 25.3%, 77.7%, 27.5%, 45.8% in plant height, leaf number, chlorophyll content and stem volume, respectively. Notably, inoculation of the strain led to a 49.0% increase in the average seed number per plant and 20% increase in the average single seed weight when plants were maintained for 1.5 years in non-sterilized soil in pots in the open air. Enterobacter sp. R4-368 cells were able to colonize root tissues and moved systemically to stem tissues. However, no bacteria were found in leaves. Promotion of plant growth and leaf nitrogen content by the strain was partially lost in nifH, nifD, nifK knockout mutants, suggesting the presence of other growth promoting factors that are associated with this bacterium strain. CONCLUSION: Our results showed that Enterobacter sp. R4-368 significantly promoted growth and seed yield of J. curcas. The application of the strains is likely to significantly improve the commercial viability of J. curcas due to the reduced fertilizer cost and improved oil yield. BioMed Central 2013-10-01 /pmc/articles/PMC3879406/ /pubmed/24083555 http://dx.doi.org/10.1186/1754-6834-6-140 Text en Copyright © 2013 Madhaiyan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Madhaiyan, Munusamy
Peng, Ni
Te, Ngoh Si
Hsin I, Cheng
Lin, Cai
Lin, Fu
Reddy, Chalapathy
Yan, Hong
Ji, Lianghui
Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species
title Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species
title_full Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species
title_fullStr Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species
title_full_unstemmed Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species
title_short Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species
title_sort improvement of plant growth and seed yield in jatropha curcas by a novel nitrogen-fixing root associated enterobacter species
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879406/
https://www.ncbi.nlm.nih.gov/pubmed/24083555
http://dx.doi.org/10.1186/1754-6834-6-140
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