Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells

As the function of transient receptor potential melastatin member 8 (TRPM8) in osteosarcoma is still unknown, we aim to investigate the possible effects and potential mechanisms of TRPM8 on cell proliferation, metastasis and chemosensitivity in osteosarcoma cells. We find that TRPM8 is aberrantly ov...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yongzhi, Yang, Zhonghua, Meng, Zhe, Cao, Hong, Zhu, Guangbin, Liu, Tao, Wang, Xinghuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2013
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879595/
https://www.ncbi.nlm.nih.gov/pubmed/24391455
http://dx.doi.org/10.7150/ijbs.7738
_version_ 1782298007442554880
author Wang, Yongzhi
Yang, Zhonghua
Meng, Zhe
Cao, Hong
Zhu, Guangbin
Liu, Tao
Wang, Xinghuan
author_facet Wang, Yongzhi
Yang, Zhonghua
Meng, Zhe
Cao, Hong
Zhu, Guangbin
Liu, Tao
Wang, Xinghuan
author_sort Wang, Yongzhi
collection PubMed
description As the function of transient receptor potential melastatin member 8 (TRPM8) in osteosarcoma is still unknown, we aim to investigate the possible effects and potential mechanisms of TRPM8 on cell proliferation, metastasis and chemosensitivity in osteosarcoma cells. We find that TRPM8 is aberrantly over-expressed in human osteosarcoma tissues and cell lines. Knockdown of TRPM8 by siRNA in osteosarcoma cells leads to the impaired regulation of intracellular Ca(2+) concentration and then the Akt-GSK-3β pathway and the phosphorylation of p44/p42 and FAK are suppressed. Knockdown of TRPM8 not only negatively influences the cell proliferation and metastasis but also enhances epirubicin-induced cell apoptosis. Such results reveal that TRPM8 is worthy further investigation for its potential as a clinical biomarker and therapeutic target in osteosarcoma.
format Online
Article
Text
id pubmed-3879595
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-38795952014-01-03 Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells Wang, Yongzhi Yang, Zhonghua Meng, Zhe Cao, Hong Zhu, Guangbin Liu, Tao Wang, Xinghuan Int J Biol Sci Research Paper As the function of transient receptor potential melastatin member 8 (TRPM8) in osteosarcoma is still unknown, we aim to investigate the possible effects and potential mechanisms of TRPM8 on cell proliferation, metastasis and chemosensitivity in osteosarcoma cells. We find that TRPM8 is aberrantly over-expressed in human osteosarcoma tissues and cell lines. Knockdown of TRPM8 by siRNA in osteosarcoma cells leads to the impaired regulation of intracellular Ca(2+) concentration and then the Akt-GSK-3β pathway and the phosphorylation of p44/p42 and FAK are suppressed. Knockdown of TRPM8 not only negatively influences the cell proliferation and metastasis but also enhances epirubicin-induced cell apoptosis. Such results reveal that TRPM8 is worthy further investigation for its potential as a clinical biomarker and therapeutic target in osteosarcoma. Ivyspring International Publisher 2013-12-22 /pmc/articles/PMC3879595/ /pubmed/24391455 http://dx.doi.org/10.7150/ijbs.7738 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Wang, Yongzhi
Yang, Zhonghua
Meng, Zhe
Cao, Hong
Zhu, Guangbin
Liu, Tao
Wang, Xinghuan
Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells
title Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells
title_full Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells
title_fullStr Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells
title_full_unstemmed Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells
title_short Knockdown of TRPM8 Suppresses Cancer Malignancy and Enhances Epirubicin-induced Apoptosis in Human Osteosarcoma Cells
title_sort knockdown of trpm8 suppresses cancer malignancy and enhances epirubicin-induced apoptosis in human osteosarcoma cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879595/
https://www.ncbi.nlm.nih.gov/pubmed/24391455
http://dx.doi.org/10.7150/ijbs.7738
work_keys_str_mv AT wangyongzhi knockdownoftrpm8suppressescancermalignancyandenhancesepirubicininducedapoptosisinhumanosteosarcomacells
AT yangzhonghua knockdownoftrpm8suppressescancermalignancyandenhancesepirubicininducedapoptosisinhumanosteosarcomacells
AT mengzhe knockdownoftrpm8suppressescancermalignancyandenhancesepirubicininducedapoptosisinhumanosteosarcomacells
AT caohong knockdownoftrpm8suppressescancermalignancyandenhancesepirubicininducedapoptosisinhumanosteosarcomacells
AT zhuguangbin knockdownoftrpm8suppressescancermalignancyandenhancesepirubicininducedapoptosisinhumanosteosarcomacells
AT liutao knockdownoftrpm8suppressescancermalignancyandenhancesepirubicininducedapoptosisinhumanosteosarcomacells
AT wangxinghuan knockdownoftrpm8suppressescancermalignancyandenhancesepirubicininducedapoptosisinhumanosteosarcomacells

Ejemplares similares