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In Vivo Imaging of Lymphatic Drainage of Cerebrospinal Fluid in Mouse

BACKGROUND: Mouse models are commonly used to study central nervous system disorders, in which cerebrospinal fluid (CSF) drainage may be disturbed. However, mouse CSF drainage into lymphatics has not been thoroughly characterized. We aimed to image this using an in vivo approach that combined quantu...

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Autores principales: Mathieu, Emily, Gupta, Neeru, Macdonald, R Loch, Ai, Jinglu, Yücel, Yeni H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879644/
https://www.ncbi.nlm.nih.gov/pubmed/24360130
http://dx.doi.org/10.1186/2045-8118-10-35
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author Mathieu, Emily
Gupta, Neeru
Macdonald, R Loch
Ai, Jinglu
Yücel, Yeni H
author_facet Mathieu, Emily
Gupta, Neeru
Macdonald, R Loch
Ai, Jinglu
Yücel, Yeni H
author_sort Mathieu, Emily
collection PubMed
description BACKGROUND: Mouse models are commonly used to study central nervous system disorders, in which cerebrospinal fluid (CSF) drainage may be disturbed. However, mouse CSF drainage into lymphatics has not been thoroughly characterized. We aimed to image this using an in vivo approach that combined quantum dot fluorescent nanoparticles with hyperspectral imaging. FINDINGS: Quantum dot 655 was injected into the CSF of the cisterna magna in seven mice and visualized by in vivo hyperspectral imaging at time points 20 and 40 min, 1, 2, and 6 h after injection. In controls (n = 4), quantum dots were applied directly onto intact dura mater covering the cisterna magna. After imaging, lymph nodes in the neck were harvested and processed post-mortem for histological analysis. After injection into the CSF, quantum dot signal was detected in vivo in submandibular lymph nodes of all mice studied as early as 20 min, but not in controls. Post-mortem gross and histological examination of lymph nodes confirmed in vivo observations. CONCLUSIONS: Non-invasive in vivo hyperspectral imaging is a useful tool to study CSF lymphatic drainage and is relevant to understanding this pathway in CNS disease models.
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spelling pubmed-38796442014-01-04 In Vivo Imaging of Lymphatic Drainage of Cerebrospinal Fluid in Mouse Mathieu, Emily Gupta, Neeru Macdonald, R Loch Ai, Jinglu Yücel, Yeni H Fluids Barriers CNS Short Paper BACKGROUND: Mouse models are commonly used to study central nervous system disorders, in which cerebrospinal fluid (CSF) drainage may be disturbed. However, mouse CSF drainage into lymphatics has not been thoroughly characterized. We aimed to image this using an in vivo approach that combined quantum dot fluorescent nanoparticles with hyperspectral imaging. FINDINGS: Quantum dot 655 was injected into the CSF of the cisterna magna in seven mice and visualized by in vivo hyperspectral imaging at time points 20 and 40 min, 1, 2, and 6 h after injection. In controls (n = 4), quantum dots were applied directly onto intact dura mater covering the cisterna magna. After imaging, lymph nodes in the neck were harvested and processed post-mortem for histological analysis. After injection into the CSF, quantum dot signal was detected in vivo in submandibular lymph nodes of all mice studied as early as 20 min, but not in controls. Post-mortem gross and histological examination of lymph nodes confirmed in vivo observations. CONCLUSIONS: Non-invasive in vivo hyperspectral imaging is a useful tool to study CSF lymphatic drainage and is relevant to understanding this pathway in CNS disease models. BioMed Central 2013-12-21 /pmc/articles/PMC3879644/ /pubmed/24360130 http://dx.doi.org/10.1186/2045-8118-10-35 Text en Copyright © 2013 Mathieu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Paper
Mathieu, Emily
Gupta, Neeru
Macdonald, R Loch
Ai, Jinglu
Yücel, Yeni H
In Vivo Imaging of Lymphatic Drainage of Cerebrospinal Fluid in Mouse
title In Vivo Imaging of Lymphatic Drainage of Cerebrospinal Fluid in Mouse
title_full In Vivo Imaging of Lymphatic Drainage of Cerebrospinal Fluid in Mouse
title_fullStr In Vivo Imaging of Lymphatic Drainage of Cerebrospinal Fluid in Mouse
title_full_unstemmed In Vivo Imaging of Lymphatic Drainage of Cerebrospinal Fluid in Mouse
title_short In Vivo Imaging of Lymphatic Drainage of Cerebrospinal Fluid in Mouse
title_sort in vivo imaging of lymphatic drainage of cerebrospinal fluid in mouse
topic Short Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879644/
https://www.ncbi.nlm.nih.gov/pubmed/24360130
http://dx.doi.org/10.1186/2045-8118-10-35
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