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Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells
Tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) was initially identified as a gene amplified and overexpressed in breast carcinoma. The present study investigated the expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells. TRAF4 was found to be localized...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881200/ https://www.ncbi.nlm.nih.gov/pubmed/24396457 http://dx.doi.org/10.3892/ol.2013.1703 |
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author | ZHANG, XIAOLI WEN, ZHIFENG MI, XIAOYI |
author_facet | ZHANG, XIAOLI WEN, ZHIFENG MI, XIAOYI |
author_sort | ZHANG, XIAOLI |
collection | PubMed |
description | Tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) was initially identified as a gene amplified and overexpressed in breast carcinoma. The present study investigated the expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells. TRAF4 was found to be localized in the cytoplasm and nuclei of MCF-7 cells by immunofluorescence staining and western blotting. The expression of TRAF4 in normal MCF-10A breast cells was found to be lower than in MCF-7 and MDA-MB-231 breast cancer cells. Following TNF-α treatment, TRAF4 depletion by siRNA in the MCF-7 cells was observed to suppress cell proliferation and the nuclear expression of nuclear factor κB was significantly reduced. The percentage of early apoptotic cells in the MCF-7 cells was augmented upon TRAF4-knockdown, and an increase in G(1) phase cells and a decrease in S phase cells was detected. These results indicate that TRAF4 has anti-apoptotic effects on apoptosis induced by TNF-α in MCF-7 cells. |
format | Online Article Text |
id | pubmed-3881200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-38812002014-01-06 Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells ZHANG, XIAOLI WEN, ZHIFENG MI, XIAOYI Oncol Lett Articles Tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) was initially identified as a gene amplified and overexpressed in breast carcinoma. The present study investigated the expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells. TRAF4 was found to be localized in the cytoplasm and nuclei of MCF-7 cells by immunofluorescence staining and western blotting. The expression of TRAF4 in normal MCF-10A breast cells was found to be lower than in MCF-7 and MDA-MB-231 breast cancer cells. Following TNF-α treatment, TRAF4 depletion by siRNA in the MCF-7 cells was observed to suppress cell proliferation and the nuclear expression of nuclear factor κB was significantly reduced. The percentage of early apoptotic cells in the MCF-7 cells was augmented upon TRAF4-knockdown, and an increase in G(1) phase cells and a decrease in S phase cells was detected. These results indicate that TRAF4 has anti-apoptotic effects on apoptosis induced by TNF-α in MCF-7 cells. D.A. Spandidos 2014-02 2013-11-25 /pmc/articles/PMC3881200/ /pubmed/24396457 http://dx.doi.org/10.3892/ol.2013.1703 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles ZHANG, XIAOLI WEN, ZHIFENG MI, XIAOYI Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells |
title | Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells |
title_full | Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells |
title_fullStr | Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells |
title_full_unstemmed | Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells |
title_short | Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF-7 cells |
title_sort | expression and anti-apoptotic function of traf4 in human breast cancer mcf-7 cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881200/ https://www.ncbi.nlm.nih.gov/pubmed/24396457 http://dx.doi.org/10.3892/ol.2013.1703 |
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