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Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene

Mycobacterium kansasii is one of the most common causes of pulmonary disease resulting from nontuberculous mycobacteria (NTM). It is also the most frequently isolated NTM species from clinical specimens in Poland. The aim of this study was to investigate the distribution of M. kansasii subtypes amon...

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Autores principales: Bakuła, Zofia, Safianowska, Aleksandra, Nowacka-Mazurek, Magdalena, Bielecki, Jacek, Jagielski, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881448/
https://www.ncbi.nlm.nih.gov/pubmed/24455675
http://dx.doi.org/10.1155/2013/178725
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author Bakuła, Zofia
Safianowska, Aleksandra
Nowacka-Mazurek, Magdalena
Bielecki, Jacek
Jagielski, Tomasz
author_facet Bakuła, Zofia
Safianowska, Aleksandra
Nowacka-Mazurek, Magdalena
Bielecki, Jacek
Jagielski, Tomasz
author_sort Bakuła, Zofia
collection PubMed
description Mycobacterium kansasii is one of the most common causes of pulmonary disease resulting from nontuberculous mycobacteria (NTM). It is also the most frequently isolated NTM species from clinical specimens in Poland. The aim of this study was to investigate the distribution of M. kansasii subtypes among patients suspected of having pulmonary NTM disease. Fifty clinical isolates of M. kansasii recovered from as many patients with suspected mycobacterial lung disease between 2000 and 2010 in Poland were genotyped by PCR-restriction enzyme analysis (PCR-REA) of partial hsp65 gene. Mycobacterium kansasii subtype I was the only genotype to be identified among the isolates, both disease-associated and non-disease-associated. Isolation of M. kansasii subtype I from clinical specimens may be indicative of infection but may also merely represent colonization.
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spelling pubmed-38814482014-01-20 Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene Bakuła, Zofia Safianowska, Aleksandra Nowacka-Mazurek, Magdalena Bielecki, Jacek Jagielski, Tomasz Biomed Res Int Research Article Mycobacterium kansasii is one of the most common causes of pulmonary disease resulting from nontuberculous mycobacteria (NTM). It is also the most frequently isolated NTM species from clinical specimens in Poland. The aim of this study was to investigate the distribution of M. kansasii subtypes among patients suspected of having pulmonary NTM disease. Fifty clinical isolates of M. kansasii recovered from as many patients with suspected mycobacterial lung disease between 2000 and 2010 in Poland were genotyped by PCR-restriction enzyme analysis (PCR-REA) of partial hsp65 gene. Mycobacterium kansasii subtype I was the only genotype to be identified among the isolates, both disease-associated and non-disease-associated. Isolation of M. kansasii subtype I from clinical specimens may be indicative of infection but may also merely represent colonization. Hindawi Publishing Corporation 2013 2013-12-22 /pmc/articles/PMC3881448/ /pubmed/24455675 http://dx.doi.org/10.1155/2013/178725 Text en Copyright © 2013 Zofia Bakuła et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bakuła, Zofia
Safianowska, Aleksandra
Nowacka-Mazurek, Magdalena
Bielecki, Jacek
Jagielski, Tomasz
Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene
title Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene
title_full Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene
title_fullStr Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene
title_full_unstemmed Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene
title_short Short Communication: Subtyping of Mycobacterium kansasii by PCR-Restriction Enzyme Analysis of the hsp65 Gene
title_sort short communication: subtyping of mycobacterium kansasii by pcr-restriction enzyme analysis of the hsp65 gene
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881448/
https://www.ncbi.nlm.nih.gov/pubmed/24455675
http://dx.doi.org/10.1155/2013/178725
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