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Identification of a Novel Garlic Cellulase Gene
Genes encoding cellulase enzymes have been investigated in various plants due to the importance of cellulase enzymes in industrial applications, especially in the conversion of biomass into biofuels. Although several cellulase genes have been cloned and characterized, little is known about cellulase...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881576/ https://www.ncbi.nlm.nih.gov/pubmed/24415833 http://dx.doi.org/10.1007/s11105-009-0159-3 |
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author | Kim, Aeri Kim, Ryong Nam Kim, Dae-Won Choi, Sang-Haeng Kang, Aram Nam, Seong-Hyeuk Park, Hong-Seog |
author_facet | Kim, Aeri Kim, Ryong Nam Kim, Dae-Won Choi, Sang-Haeng Kang, Aram Nam, Seong-Hyeuk Park, Hong-Seog |
author_sort | Kim, Aeri |
collection | PubMed |
description | Genes encoding cellulase enzymes have been investigated in various plants due to the importance of cellulase enzymes in industrial applications, especially in the conversion of biomass into biofuels. Although several cellulase genes have been cloned and characterized, little is known about cellulase genes from garlic or enzyme activities of their gene products. In this study, a cellulase gene from garlic was cloned and characterized in gene and protein levels for the first time. The DNA sequence of the garlic cellulase gene showed 81% identity with the sequence of the endo-beta-1,4-glucanase of Pisum sativum. The open reading frame of this gene is 1,506 bp, which corresponds to 501 deduced amino acids. We identified the novel ORF region, which was translated into a 55.2 kDa protein using the protein expression vector, pET28a, in Escherichia coli and we confirmed that this protein has cellulase activity in vitro. Our study demonstrates that garlic is very useful, not only for the culinary and pharmaceutical industries, but also as an excellent natural source of various kinds of important genes and enzymes. |
format | Online Article Text |
id | pubmed-3881576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-38815762014-01-08 Identification of a Novel Garlic Cellulase Gene Kim, Aeri Kim, Ryong Nam Kim, Dae-Won Choi, Sang-Haeng Kang, Aram Nam, Seong-Hyeuk Park, Hong-Seog Plant Mol Biol Report Article Genes encoding cellulase enzymes have been investigated in various plants due to the importance of cellulase enzymes in industrial applications, especially in the conversion of biomass into biofuels. Although several cellulase genes have been cloned and characterized, little is known about cellulase genes from garlic or enzyme activities of their gene products. In this study, a cellulase gene from garlic was cloned and characterized in gene and protein levels for the first time. The DNA sequence of the garlic cellulase gene showed 81% identity with the sequence of the endo-beta-1,4-glucanase of Pisum sativum. The open reading frame of this gene is 1,506 bp, which corresponds to 501 deduced amino acids. We identified the novel ORF region, which was translated into a 55.2 kDa protein using the protein expression vector, pET28a, in Escherichia coli and we confirmed that this protein has cellulase activity in vitro. Our study demonstrates that garlic is very useful, not only for the culinary and pharmaceutical industries, but also as an excellent natural source of various kinds of important genes and enzymes. Springer-Verlag 2010-01-07 2010 /pmc/articles/PMC3881576/ /pubmed/24415833 http://dx.doi.org/10.1007/s11105-009-0159-3 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Kim, Aeri Kim, Ryong Nam Kim, Dae-Won Choi, Sang-Haeng Kang, Aram Nam, Seong-Hyeuk Park, Hong-Seog Identification of a Novel Garlic Cellulase Gene |
title | Identification of a Novel Garlic Cellulase Gene |
title_full | Identification of a Novel Garlic Cellulase Gene |
title_fullStr | Identification of a Novel Garlic Cellulase Gene |
title_full_unstemmed | Identification of a Novel Garlic Cellulase Gene |
title_short | Identification of a Novel Garlic Cellulase Gene |
title_sort | identification of a novel garlic cellulase gene |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881576/ https://www.ncbi.nlm.nih.gov/pubmed/24415833 http://dx.doi.org/10.1007/s11105-009-0159-3 |
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