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Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants

Production of recombinant proteins in plants is of increasing importance for practical applications. However, the production of stable transformed transgenic plants is a lengthy procedure. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors...

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Autores principales: Shah, Kausar Hussain, Almaghrabi, Bachar, Bohlmann, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881577/
https://www.ncbi.nlm.nih.gov/pubmed/24415845
http://dx.doi.org/10.1007/s11105-013-0614-z
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author Shah, Kausar Hussain
Almaghrabi, Bachar
Bohlmann, Holger
author_facet Shah, Kausar Hussain
Almaghrabi, Bachar
Bohlmann, Holger
author_sort Shah, Kausar Hussain
collection PubMed
description Production of recombinant proteins in plants is of increasing importance for practical applications. However, the production of stable transformed transgenic plants is a lengthy procedure. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors have been constructed for that purpose. Each of them is reported to be highly efficient, robust and cost-effective. Here, a variety of expression vectors which were designed for transient and stable plant transformation, including pPZP3425, pPZP5025, pPZPTRBO, pJLTRBO, pEAQ-HT and pBY030-2R, was compared for the expression of green fluorescent protein and β-glucuronidase in Nicotiana benthamiana by Agrobacterium-mediated transient expression. Our results show that pPZPTRBO, pJLTRBO and pEAQ-HT had comparable expression levels without co-infiltration of a RNA-silencing inhibitor. The other vectors, including the non-viral vectors pPZP5025 and pPZP3425, needed co-infiltration of the RNA-silencing inhibitor P19 to give good expression levels. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11105-013-0614-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-38815772014-01-08 Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants Shah, Kausar Hussain Almaghrabi, Bachar Bohlmann, Holger Plant Mol Biol Report Original Paper Production of recombinant proteins in plants is of increasing importance for practical applications. However, the production of stable transformed transgenic plants is a lengthy procedure. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors have been constructed for that purpose. Each of them is reported to be highly efficient, robust and cost-effective. Here, a variety of expression vectors which were designed for transient and stable plant transformation, including pPZP3425, pPZP5025, pPZPTRBO, pJLTRBO, pEAQ-HT and pBY030-2R, was compared for the expression of green fluorescent protein and β-glucuronidase in Nicotiana benthamiana by Agrobacterium-mediated transient expression. Our results show that pPZPTRBO, pJLTRBO and pEAQ-HT had comparable expression levels without co-infiltration of a RNA-silencing inhibitor. The other vectors, including the non-viral vectors pPZP5025 and pPZP3425, needed co-infiltration of the RNA-silencing inhibitor P19 to give good expression levels. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11105-013-0614-z) contains supplementary material, which is available to authorized users. Springer US 2013-07-25 2013 /pmc/articles/PMC3881577/ /pubmed/24415845 http://dx.doi.org/10.1007/s11105-013-0614-z Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Shah, Kausar Hussain
Almaghrabi, Bachar
Bohlmann, Holger
Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants
title Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants
title_full Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants
title_fullStr Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants
title_full_unstemmed Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants
title_short Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants
title_sort comparison of expression vectors for transient expression of recombinant proteins in plants
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881577/
https://www.ncbi.nlm.nih.gov/pubmed/24415845
http://dx.doi.org/10.1007/s11105-013-0614-z
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