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Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line

In the present study, to identify the effective components of Chinese traditional herbs, Euphorbia hylonoma Hand.-Mazz. (Euphorbiaceae), a folk herb that has been used among the Qinling mountain area for hundreds of years, was investigated. 3,3′-Di-O-methyl ellagic acid-4′-O-β-d-xylopyranoside (JNE2...

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Autores principales: ZHANG, HUI, GUO, ZENG-JUN, XU, WEN-MING, YOU, XIAO-JUAN, HAN, LING, HAN, YAN-XIA, DAI, LIU-JIANG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881749/
https://www.ncbi.nlm.nih.gov/pubmed/24396481
http://dx.doi.org/10.3892/ol.2013.1740
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author ZHANG, HUI
GUO, ZENG-JUN
XU, WEN-MING
YOU, XIAO-JUAN
HAN, LING
HAN, YAN-XIA
DAI, LIU-JIANG
author_facet ZHANG, HUI
GUO, ZENG-JUN
XU, WEN-MING
YOU, XIAO-JUAN
HAN, LING
HAN, YAN-XIA
DAI, LIU-JIANG
author_sort ZHANG, HUI
collection PubMed
description In the present study, to identify the effective components of Chinese traditional herbs, Euphorbia hylonoma Hand.-Mazz. (Euphorbiaceae), a folk herb that has been used among the Qinling mountain area for hundreds of years, was investigated. 3,3′-Di-O-methyl ellagic acid-4′-O-β-d-xylopyranoside (JNE2), an ellagic acid derivative, was isolated from the acetone extract of the herb and its antitumor activity against human hepatoma HepG2 cells was detected in vitro. The results showed that JNE2 inhibited the proliferation of HepG2 cells in a dose- and time-dependent manner and blocked the cell cycle at the G1/S phase. A high dosage of JNE2 induced apoptosis of the tumor cells, but no significant differences were identified between the treatment groups. The invasiveness of HepG2 cells was also inhibited by JNE2. The mechanism of the antitumor effect of JNE2 at the molecular level was presumed to be due to the upregulation of the protein expression of Bax and caspase-3, and the downregulation of the protein expression of Bcl-2 and CCND1. The results suggested that JNE2 is a potential antitumor agent that merits further investigation.
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spelling pubmed-38817492014-01-06 Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line ZHANG, HUI GUO, ZENG-JUN XU, WEN-MING YOU, XIAO-JUAN HAN, LING HAN, YAN-XIA DAI, LIU-JIANG Oncol Lett Articles In the present study, to identify the effective components of Chinese traditional herbs, Euphorbia hylonoma Hand.-Mazz. (Euphorbiaceae), a folk herb that has been used among the Qinling mountain area for hundreds of years, was investigated. 3,3′-Di-O-methyl ellagic acid-4′-O-β-d-xylopyranoside (JNE2), an ellagic acid derivative, was isolated from the acetone extract of the herb and its antitumor activity against human hepatoma HepG2 cells was detected in vitro. The results showed that JNE2 inhibited the proliferation of HepG2 cells in a dose- and time-dependent manner and blocked the cell cycle at the G1/S phase. A high dosage of JNE2 induced apoptosis of the tumor cells, but no significant differences were identified between the treatment groups. The invasiveness of HepG2 cells was also inhibited by JNE2. The mechanism of the antitumor effect of JNE2 at the molecular level was presumed to be due to the upregulation of the protein expression of Bax and caspase-3, and the downregulation of the protein expression of Bcl-2 and CCND1. The results suggested that JNE2 is a potential antitumor agent that merits further investigation. D.A. Spandidos 2014-02 2013-12-06 /pmc/articles/PMC3881749/ /pubmed/24396481 http://dx.doi.org/10.3892/ol.2013.1740 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ZHANG, HUI
GUO, ZENG-JUN
XU, WEN-MING
YOU, XIAO-JUAN
HAN, LING
HAN, YAN-XIA
DAI, LIU-JIANG
Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line
title Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line
title_full Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line
title_fullStr Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line
title_full_unstemmed Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line
title_short Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line
title_sort antitumor effect and mechanism of an ellagic acid derivative on the hepg2 human hepatocellular carcinoma cell line
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881749/
https://www.ncbi.nlm.nih.gov/pubmed/24396481
http://dx.doi.org/10.3892/ol.2013.1740
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