Multiplexed MS/MS for Improved Data Independent Acquisition

In mass spectrometry based proteomics, data-independent acquisition (DIA) strategies have the ability to acquire a single dataset useful for identification and quantification of detectable peptides in a complex mixture. Despite this, DIA is often overlooked due to noisier data resulting from a typic...

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Detalles Bibliográficos
Autores principales: Egertson, Jarrett D., Kuehn, Andreas, Merrihew, Gennifer E., Bateman, Nicholas W., MacLean, Brendan X., Ting, Ying S., Canterbury, Jesse D., Marsh, Donald M., Kellmann, Markus, Zabrouskov, Vlad, Wu, Christine C., MacCoss, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3881977/
https://www.ncbi.nlm.nih.gov/pubmed/23793237
http://dx.doi.org/10.1038/nmeth.2528
Descripción
Sumario:In mass spectrometry based proteomics, data-independent acquisition (DIA) strategies have the ability to acquire a single dataset useful for identification and quantification of detectable peptides in a complex mixture. Despite this, DIA is often overlooked due to noisier data resulting from a typical five to ten fold reduction in precursor selectivity compared to data dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing technique which improves precursor selectivity five-fold.

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