Cargando…

Lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts Mi-flp-18 expression to block infection

Tomato (Solanum lycopersicum) crops can be severely damaged due to parasitism by the root-knot nematode (RKN) Meloidogyne incognita, but are protected when intercropped with crown daisy (Chrysanthemum coronarium L.). Root exudate may be the determining factor for this protection. An experiment using...

Descripción completa

Detalles Bibliográficos
Autores principales: Dong, Linlin, Li, Xiaolin, Huang, Li, Gao, Ying, Zhong, Lina, Zheng, Yuanyuan, Zuo, Yuanmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3883285/
https://www.ncbi.nlm.nih.gov/pubmed/24170741
http://dx.doi.org/10.1093/jxb/ert356
_version_ 1782298431680675840
author Dong, Linlin
Li, Xiaolin
Huang, Li
Gao, Ying
Zhong, Lina
Zheng, Yuanyuan
Zuo, Yuanmei
author_facet Dong, Linlin
Li, Xiaolin
Huang, Li
Gao, Ying
Zhong, Lina
Zheng, Yuanyuan
Zuo, Yuanmei
author_sort Dong, Linlin
collection PubMed
description Tomato (Solanum lycopersicum) crops can be severely damaged due to parasitism by the root-knot nematode (RKN) Meloidogyne incognita, but are protected when intercropped with crown daisy (Chrysanthemum coronarium L.). Root exudate may be the determining factor for this protection. An experiment using pots linked by a tube and Petri dish experiments were undertaken to confirm that tomato–crown daisy intercropping root exudate decreased the number of nematodes and alleviated nematode damage, and to determine crown daisy root exudate-regulated nematode chemotaxis. Following a gas chromatography–mass spectrometry assay, it was found that the intercropping protection was derived from the potent bioactivity of a specific root exudate component of crown daisy, namely lauric acid. The Mi-flp-18 gene, encoding an FMRFamide-like peptide neuromodulator, regulated nematode chemotaxis and infection by RNA interference. Moreover, it was shown that lauric acid acts as both a lethal trap and a repellent for M. incognita by specifically regulating Mi-flp-18 expression in a concentration-dependent manner. Low concentrations of lauric acid (0.5–2.0mM) attract M. incognita and consequently cause death, while high concentrations (4.0mM) repel M. incognita. This study elucidates how lauric acid in crown daisy root exudate regulates nematode chemotaxis and disrupts Mi-flp-18 expression to alleviate nematode damage, and presents a general methodology for studying signalling systems affected by plant root exudates in the rhizosphere. This could lead to the development of economical and feasible strategies for controlling plant-parasitic nematodes, and provide an alternative to the use of pesticides in farming systems.
format Online
Article
Text
id pubmed-3883285
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-38832852014-01-07 Lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts Mi-flp-18 expression to block infection Dong, Linlin Li, Xiaolin Huang, Li Gao, Ying Zhong, Lina Zheng, Yuanyuan Zuo, Yuanmei J Exp Bot Research Paper Tomato (Solanum lycopersicum) crops can be severely damaged due to parasitism by the root-knot nematode (RKN) Meloidogyne incognita, but are protected when intercropped with crown daisy (Chrysanthemum coronarium L.). Root exudate may be the determining factor for this protection. An experiment using pots linked by a tube and Petri dish experiments were undertaken to confirm that tomato–crown daisy intercropping root exudate decreased the number of nematodes and alleviated nematode damage, and to determine crown daisy root exudate-regulated nematode chemotaxis. Following a gas chromatography–mass spectrometry assay, it was found that the intercropping protection was derived from the potent bioactivity of a specific root exudate component of crown daisy, namely lauric acid. The Mi-flp-18 gene, encoding an FMRFamide-like peptide neuromodulator, regulated nematode chemotaxis and infection by RNA interference. Moreover, it was shown that lauric acid acts as both a lethal trap and a repellent for M. incognita by specifically regulating Mi-flp-18 expression in a concentration-dependent manner. Low concentrations of lauric acid (0.5–2.0mM) attract M. incognita and consequently cause death, while high concentrations (4.0mM) repel M. incognita. This study elucidates how lauric acid in crown daisy root exudate regulates nematode chemotaxis and disrupts Mi-flp-18 expression to alleviate nematode damage, and presents a general methodology for studying signalling systems affected by plant root exudates in the rhizosphere. This could lead to the development of economical and feasible strategies for controlling plant-parasitic nematodes, and provide an alternative to the use of pesticides in farming systems. Oxford University Press 2014-01 2013-10-29 /pmc/articles/PMC3883285/ /pubmed/24170741 http://dx.doi.org/10.1093/jxb/ert356 Text en © The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Dong, Linlin
Li, Xiaolin
Huang, Li
Gao, Ying
Zhong, Lina
Zheng, Yuanyuan
Zuo, Yuanmei
Lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts Mi-flp-18 expression to block infection
title Lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts Mi-flp-18 expression to block infection
title_full Lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts Mi-flp-18 expression to block infection
title_fullStr Lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts Mi-flp-18 expression to block infection
title_full_unstemmed Lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts Mi-flp-18 expression to block infection
title_short Lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts Mi-flp-18 expression to block infection
title_sort lauric acid in crown daisy root exudate potently regulates root-knot nematode chemotaxis and disrupts mi-flp-18 expression to block infection
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3883285/
https://www.ncbi.nlm.nih.gov/pubmed/24170741
http://dx.doi.org/10.1093/jxb/ert356
work_keys_str_mv AT donglinlin lauricacidincrowndaisyrootexudatepotentlyregulatesrootknotnematodechemotaxisanddisruptsmiflp18expressiontoblockinfection
AT lixiaolin lauricacidincrowndaisyrootexudatepotentlyregulatesrootknotnematodechemotaxisanddisruptsmiflp18expressiontoblockinfection
AT huangli lauricacidincrowndaisyrootexudatepotentlyregulatesrootknotnematodechemotaxisanddisruptsmiflp18expressiontoblockinfection
AT gaoying lauricacidincrowndaisyrootexudatepotentlyregulatesrootknotnematodechemotaxisanddisruptsmiflp18expressiontoblockinfection
AT zhonglina lauricacidincrowndaisyrootexudatepotentlyregulatesrootknotnematodechemotaxisanddisruptsmiflp18expressiontoblockinfection
AT zhengyuanyuan lauricacidincrowndaisyrootexudatepotentlyregulatesrootknotnematodechemotaxisanddisruptsmiflp18expressiontoblockinfection
AT zuoyuanmei lauricacidincrowndaisyrootexudatepotentlyregulatesrootknotnematodechemotaxisanddisruptsmiflp18expressiontoblockinfection