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Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system

Haploid embryonic stem cells (ESCs) are useful for studying mammalian genes because disruption of only one allele can cause loss-of-function phenotypes. Here, we report the use of haploid ESCs and the CRISPR RNA-guided Cas9 nuclease gene-targeting system to manipulate mammalian genes. Co-transfectio...

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Detalles Bibliográficos
Autores principales:	Horii, Takuro, Morita, Sumiyo, Kimura, Mika, Kobayashi, Ryouhei, Tamura, Daiki, Takahashi, Ryou-u, Kimura, Hironobu, Suetake, Isao, Ohata, Hirokazu, Okamoto, Koji, Tajima, Shoji, Ochiya, Takahiro, Abe, Yumiko, Hatada, Izuho
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	PeerJ Inc. 2013
Materias:	Developmental Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3883491/ https://www.ncbi.nlm.nih.gov/pubmed/24432195 http://dx.doi.org/10.7717/peerj.230

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