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Functional metagenomic profiling of intestinal microbiome in extreme ageing
Age-related alterations in human gut microbiota composition have been thoroughly described, but a detailed functional description of the intestinal bacterial coding capacity is still missing. In order to elucidate the contribution of the gut metagenome to the complex mosaic of human longevity, we ap...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3883706/ https://www.ncbi.nlm.nih.gov/pubmed/24334635 |
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author | Rampelli, Simone Candela, Marco Turroni, Silvia Biagi, Elena Collino, Sebastiano Franceschi, Claudio O'Toole, Paul W Brigidi, Patrizia |
author_facet | Rampelli, Simone Candela, Marco Turroni, Silvia Biagi, Elena Collino, Sebastiano Franceschi, Claudio O'Toole, Paul W Brigidi, Patrizia |
author_sort | Rampelli, Simone |
collection | PubMed |
description | Age-related alterations in human gut microbiota composition have been thoroughly described, but a detailed functional description of the intestinal bacterial coding capacity is still missing. In order to elucidate the contribution of the gut metagenome to the complex mosaic of human longevity, we applied shotgun sequencing to total fecal bacterial DNA in a selection of samples belonging to a well-characterized human ageing cohort. The age-related trajectory of the human gut microbiome was characterized by loss of genes for shortchain fatty acid production and an overall decrease in the saccharolytic potential, while proteolytic functions were more abundant than in the intestinal metagenome of younger adults. This altered functional profile was associated with a relevant enrichment in “pathobionts”, i.e. opportunistic pro-inflammatory bacteria generally present in the adult gut ecosystem in low numbers. Finally, as a signature for long life we identified 116 microbial genes that significantly correlated with ageing. Collectively, our data emphasize the relationship between intestinal bacteria and human metabolism, by detailing the modifications in the gut microbiota as a consequence of and/or promoter of the physiological changes occurring in the human host upon ageing. |
format | Online Article Text |
id | pubmed-3883706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-38837062014-01-13 Functional metagenomic profiling of intestinal microbiome in extreme ageing Rampelli, Simone Candela, Marco Turroni, Silvia Biagi, Elena Collino, Sebastiano Franceschi, Claudio O'Toole, Paul W Brigidi, Patrizia Aging (Albany NY) Research Paper Age-related alterations in human gut microbiota composition have been thoroughly described, but a detailed functional description of the intestinal bacterial coding capacity is still missing. In order to elucidate the contribution of the gut metagenome to the complex mosaic of human longevity, we applied shotgun sequencing to total fecal bacterial DNA in a selection of samples belonging to a well-characterized human ageing cohort. The age-related trajectory of the human gut microbiome was characterized by loss of genes for shortchain fatty acid production and an overall decrease in the saccharolytic potential, while proteolytic functions were more abundant than in the intestinal metagenome of younger adults. This altered functional profile was associated with a relevant enrichment in “pathobionts”, i.e. opportunistic pro-inflammatory bacteria generally present in the adult gut ecosystem in low numbers. Finally, as a signature for long life we identified 116 microbial genes that significantly correlated with ageing. Collectively, our data emphasize the relationship between intestinal bacteria and human metabolism, by detailing the modifications in the gut microbiota as a consequence of and/or promoter of the physiological changes occurring in the human host upon ageing. Impact Journals LLC 2013-12-10 /pmc/articles/PMC3883706/ /pubmed/24334635 Text en Copyright: © 2013 Rampelli et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
spellingShingle | Research Paper Rampelli, Simone Candela, Marco Turroni, Silvia Biagi, Elena Collino, Sebastiano Franceschi, Claudio O'Toole, Paul W Brigidi, Patrizia Functional metagenomic profiling of intestinal microbiome in extreme ageing |
title | Functional metagenomic profiling of intestinal microbiome in extreme ageing |
title_full | Functional metagenomic profiling of intestinal microbiome in extreme ageing |
title_fullStr | Functional metagenomic profiling of intestinal microbiome in extreme ageing |
title_full_unstemmed | Functional metagenomic profiling of intestinal microbiome in extreme ageing |
title_short | Functional metagenomic profiling of intestinal microbiome in extreme ageing |
title_sort | functional metagenomic profiling of intestinal microbiome in extreme ageing |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3883706/ https://www.ncbi.nlm.nih.gov/pubmed/24334635 |
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