LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isol...

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Detalles Bibliográficos
Autores principales: Horník, Štěpán, Sajfrtová, Marie, Karban, Jindřich, Sýkora, Jan, Březinová, Anna, Wimmer, Zdeněk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3884959/
https://www.ncbi.nlm.nih.gov/pubmed/24455424
http://dx.doi.org/10.1155/2013/526818
Descripción
Sumario:The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.

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