LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isol...

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Detalles Bibliográficos
Autores principales: Horník, Štěpán, Sajfrtová, Marie, Karban, Jindřich, Sýkora, Jan, Březinová, Anna, Wimmer, Zdeněk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3884959/
https://www.ncbi.nlm.nih.gov/pubmed/24455424
http://dx.doi.org/10.1155/2013/526818
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author Horník, Štěpán
Sajfrtová, Marie
Karban, Jindřich
Sýkora, Jan
Březinová, Anna
Wimmer, Zdeněk
author_facet Horník, Štěpán
Sajfrtová, Marie
Karban, Jindřich
Sýkora, Jan
Březinová, Anna
Wimmer, Zdeněk
author_sort Horník, Štěpán
collection PubMed
description The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data.
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spelling pubmed-38849592014-01-21 LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts Horník, Štěpán Sajfrtová, Marie Karban, Jindřich Sýkora, Jan Březinová, Anna Wimmer, Zdeněk J Anal Methods Chem Research Article The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods. The results of qualitative and quantitative analyses were in a good agreement with the literature data. Hindawi Publishing Corporation 2013 2013-12-23 /pmc/articles/PMC3884959/ /pubmed/24455424 http://dx.doi.org/10.1155/2013/526818 Text en Copyright © 2013 Štěpán Horník et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Horník, Štěpán
Sajfrtová, Marie
Karban, Jindřich
Sýkora, Jan
Březinová, Anna
Wimmer, Zdeněk
LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
title LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
title_full LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
title_fullStr LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
title_full_unstemmed LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
title_short LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
title_sort lc-nmr technique in the analysis of phytosterols in natural extracts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3884959/
https://www.ncbi.nlm.nih.gov/pubmed/24455424
http://dx.doi.org/10.1155/2013/526818
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