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Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network

Recent studies indicate that propofol may protect cells via suppressing autophagic cell death caused by excessive reactive oxygen species induced by hypoxia reoxygenation (H/R). It is established that gene expression patterns including autophagy-related genes changed significantly during the process...

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Autores principales: Chen, Zhuo, Hu, Zhe, Lu, Zhiqi, Cai, Shuyun, Gu, Xiaoxia, Zhuang, Haixia, Ruan, Zhihua, Xia, Zhengyuan, Irwin, Michael G., Feng, Du, Zhang, Liangqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885199/
https://www.ncbi.nlm.nih.gov/pubmed/24454982
http://dx.doi.org/10.1155/2013/378484
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author Chen, Zhuo
Hu, Zhe
Lu, Zhiqi
Cai, Shuyun
Gu, Xiaoxia
Zhuang, Haixia
Ruan, Zhihua
Xia, Zhengyuan
Irwin, Michael G.
Feng, Du
Zhang, Liangqing
author_facet Chen, Zhuo
Hu, Zhe
Lu, Zhiqi
Cai, Shuyun
Gu, Xiaoxia
Zhuang, Haixia
Ruan, Zhihua
Xia, Zhengyuan
Irwin, Michael G.
Feng, Du
Zhang, Liangqing
author_sort Chen, Zhuo
collection PubMed
description Recent studies indicate that propofol may protect cells via suppressing autophagic cell death caused by excessive reactive oxygen species induced by hypoxia reoxygenation (H/R). It is established that gene expression patterns including autophagy-related genes changed significantly during the process of H/R in the presence or absence of propofol posthypoxia treatment (P-PostH). The reasons for such differences, however, remain largely unknown. MicroRNAs provide a novel mechanism for gene regulation. In the present study, we systematically analyzed the alterations in microRNA expression using human umbilical vein endothelial cells (HUVECs) subjected to H/R in the presence or absence of posthypoxic propofol treatment. Genome-wide profiling of microRNAs was then conducted using microRNA microarray. Fourteen miRNAs are differentially expressed and six of them were validated by the quantitative real-time PCR (Q-PCR) of which three were substantially increased, whereas one was decreased. To gain an unbiased global perspective on subsequent regulation by altered miRNAs, predicted targets of ten miRNAs were analyzed using the Gene Ontology (GO) analysis to build signaling networks. Interestingly, six of the identified microRNAs are known to target autophagy-related genes. In conclusion, our results revealed that different miRNA expression patterns are induced by propofol posthypoxia treatment in H/R and the alterations in miRNA expression patterns are implicated in regulating distinctive autophagy-related gene expression.
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spelling pubmed-38851992014-01-21 Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network Chen, Zhuo Hu, Zhe Lu, Zhiqi Cai, Shuyun Gu, Xiaoxia Zhuang, Haixia Ruan, Zhihua Xia, Zhengyuan Irwin, Michael G. Feng, Du Zhang, Liangqing Oxid Med Cell Longev Research Article Recent studies indicate that propofol may protect cells via suppressing autophagic cell death caused by excessive reactive oxygen species induced by hypoxia reoxygenation (H/R). It is established that gene expression patterns including autophagy-related genes changed significantly during the process of H/R in the presence or absence of propofol posthypoxia treatment (P-PostH). The reasons for such differences, however, remain largely unknown. MicroRNAs provide a novel mechanism for gene regulation. In the present study, we systematically analyzed the alterations in microRNA expression using human umbilical vein endothelial cells (HUVECs) subjected to H/R in the presence or absence of posthypoxic propofol treatment. Genome-wide profiling of microRNAs was then conducted using microRNA microarray. Fourteen miRNAs are differentially expressed and six of them were validated by the quantitative real-time PCR (Q-PCR) of which three were substantially increased, whereas one was decreased. To gain an unbiased global perspective on subsequent regulation by altered miRNAs, predicted targets of ten miRNAs were analyzed using the Gene Ontology (GO) analysis to build signaling networks. Interestingly, six of the identified microRNAs are known to target autophagy-related genes. In conclusion, our results revealed that different miRNA expression patterns are induced by propofol posthypoxia treatment in H/R and the alterations in miRNA expression patterns are implicated in regulating distinctive autophagy-related gene expression. Hindawi Publishing Corporation 2013 2013-12-22 /pmc/articles/PMC3885199/ /pubmed/24454982 http://dx.doi.org/10.1155/2013/378484 Text en Copyright © 2013 Zhuo Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Zhuo
Hu, Zhe
Lu, Zhiqi
Cai, Shuyun
Gu, Xiaoxia
Zhuang, Haixia
Ruan, Zhihua
Xia, Zhengyuan
Irwin, Michael G.
Feng, Du
Zhang, Liangqing
Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
title Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
title_full Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
title_fullStr Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
title_full_unstemmed Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
title_short Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
title_sort differential microrna profiling in a cellular hypoxia reoxygenation model upon posthypoxic propofol treatment reveals alterations in autophagy signaling network
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885199/
https://www.ncbi.nlm.nih.gov/pubmed/24454982
http://dx.doi.org/10.1155/2013/378484
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