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In Vitro and In Vivo Antimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders

Based on the collected ethnobotanical data from the Traditional Medicine and Materia Medica Research Center (TMRC), Iran, Myrtus communis L. (myrtle) was selected for the assessment of in vitro and in vivo antimalarial and cytotoxic activities. Methanolic extract of myrtle was prepared from the aeri...

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Autores principales: Naghibi, Farzaneh, Esmaeili, Somayeh, Abdullah, Noor Rain, Nateghpour, Mehdi, Taghvai, Mahdieh, Kamkar, Siamak, Mosaddegh, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885200/
https://www.ncbi.nlm.nih.gov/pubmed/24455686
http://dx.doi.org/10.1155/2013/316185
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author Naghibi, Farzaneh
Esmaeili, Somayeh
Abdullah, Noor Rain
Nateghpour, Mehdi
Taghvai, Mahdieh
Kamkar, Siamak
Mosaddegh, Mahmoud
author_facet Naghibi, Farzaneh
Esmaeili, Somayeh
Abdullah, Noor Rain
Nateghpour, Mehdi
Taghvai, Mahdieh
Kamkar, Siamak
Mosaddegh, Mahmoud
author_sort Naghibi, Farzaneh
collection PubMed
description Based on the collected ethnobotanical data from the Traditional Medicine and Materia Medica Research Center (TMRC), Iran, Myrtus communis L. (myrtle) was selected for the assessment of in vitro and in vivo antimalarial and cytotoxic activities. Methanolic extract of myrtle was prepared from the aerial parts and assessed for antiplasmodial activity, using the parasite lactate dehydrogenase (pLDH) assay against chloroquine-resistant (K1) and chloroquine-sensitive (3D7) strains of Plasmodium falciparum. The 4-day suppressive test was employed to determine the parasitemia suppression of the myrtle extract against P. berghei   in vivo. The IC(50) values of myrtle extract were 35.44 µg/ml against K1 and 0.87 µg/ml against 3D7. Myrtle extract showed a significant suppression of parasitaemia (84.8 ± 1.1% at 10 mg/kg/day) in mice infected with P. berghei after 4 days of treatment. Cytotoxic activity was carried out against mammalian cell lines using methyl thiazol tetrazolium (MTT) assay. No cytotoxic effect on mammalian cell lines up to 100 µg/mL was shown. The results support the traditional use of myrtle in malaria. Phytochemical investigation and understanding the mechanism of action would be in our upcoming project.
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spelling pubmed-38852002014-01-20 In Vitro and In Vivo Antimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders Naghibi, Farzaneh Esmaeili, Somayeh Abdullah, Noor Rain Nateghpour, Mehdi Taghvai, Mahdieh Kamkar, Siamak Mosaddegh, Mahmoud Biomed Res Int Research Article Based on the collected ethnobotanical data from the Traditional Medicine and Materia Medica Research Center (TMRC), Iran, Myrtus communis L. (myrtle) was selected for the assessment of in vitro and in vivo antimalarial and cytotoxic activities. Methanolic extract of myrtle was prepared from the aerial parts and assessed for antiplasmodial activity, using the parasite lactate dehydrogenase (pLDH) assay against chloroquine-resistant (K1) and chloroquine-sensitive (3D7) strains of Plasmodium falciparum. The 4-day suppressive test was employed to determine the parasitemia suppression of the myrtle extract against P. berghei   in vivo. The IC(50) values of myrtle extract were 35.44 µg/ml against K1 and 0.87 µg/ml against 3D7. Myrtle extract showed a significant suppression of parasitaemia (84.8 ± 1.1% at 10 mg/kg/day) in mice infected with P. berghei after 4 days of treatment. Cytotoxic activity was carried out against mammalian cell lines using methyl thiazol tetrazolium (MTT) assay. No cytotoxic effect on mammalian cell lines up to 100 µg/mL was shown. The results support the traditional use of myrtle in malaria. Phytochemical investigation and understanding the mechanism of action would be in our upcoming project. Hindawi Publishing Corporation 2013 2013-12-23 /pmc/articles/PMC3885200/ /pubmed/24455686 http://dx.doi.org/10.1155/2013/316185 Text en Copyright © 2013 Farzaneh Naghibi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Naghibi, Farzaneh
Esmaeili, Somayeh
Abdullah, Noor Rain
Nateghpour, Mehdi
Taghvai, Mahdieh
Kamkar, Siamak
Mosaddegh, Mahmoud
In Vitro and In Vivo Antimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders
title In Vitro and In Vivo Antimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders
title_full In Vitro and In Vivo Antimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders
title_fullStr In Vitro and In Vivo Antimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders
title_full_unstemmed In Vitro and In Vivo Antimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders
title_short In Vitro and In Vivo Antimalarial Evaluations of Myrtle Extract, a Plant Traditionally Used for Treatment of Parasitic Disorders
title_sort in vitro and in vivo antimalarial evaluations of myrtle extract, a plant traditionally used for treatment of parasitic disorders
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885200/
https://www.ncbi.nlm.nih.gov/pubmed/24455686
http://dx.doi.org/10.1155/2013/316185
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