Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reactio...

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Detalles Bibliográficos
Autores principales: García, Alfredo, Martínez, Remigio, Benitez-Medina, José Manuel, Risco, David, García, Waldo Luis, Rey, Joaquín, Alonso, Juan Manuel, de Mendoza, Javier Hermoso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885745/
https://www.ncbi.nlm.nih.gov/pubmed/23820221
http://dx.doi.org/10.4142/jvs.2013.14.4.491
Descripción
Sumario:Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.

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