Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reactio...

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Autores principales: García, Alfredo, Martínez, Remigio, Benitez-Medina, José Manuel, Risco, David, García, Waldo Luis, Rey, Joaquín, Alonso, Juan Manuel, de Mendoza, Javier Hermoso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2013
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885745/
https://www.ncbi.nlm.nih.gov/pubmed/23820221
http://dx.doi.org/10.4142/jvs.2013.14.4.491
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author García, Alfredo
Martínez, Remigio
Benitez-Medina, José Manuel
Risco, David
García, Waldo Luis
Rey, Joaquín
Alonso, Juan Manuel
de Mendoza, Javier Hermoso
author_facet García, Alfredo
Martínez, Remigio
Benitez-Medina, José Manuel
Risco, David
García, Waldo Luis
Rey, Joaquín
Alonso, Juan Manuel
de Mendoza, Javier Hermoso
author_sort García, Alfredo
collection PubMed
description Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
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spelling pubmed-38857452014-01-13 Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis García, Alfredo Martínez, Remigio Benitez-Medina, José Manuel Risco, David García, Waldo Luis Rey, Joaquín Alonso, Juan Manuel de Mendoza, Javier Hermoso J Vet Sci Short Communication Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis. The Korean Society of Veterinary Science 2013-12 2013-12-19 /pmc/articles/PMC3885745/ /pubmed/23820221 http://dx.doi.org/10.4142/jvs.2013.14.4.491 Text en © 2013 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
García, Alfredo
Martínez, Remigio
Benitez-Medina, José Manuel
Risco, David
García, Waldo Luis
Rey, Joaquín
Alonso, Juan Manuel
de Mendoza, Javier Hermoso
Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
title Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
title_full Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
title_fullStr Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
title_full_unstemmed Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
title_short Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
title_sort development of a real-time sybr green pcr assay for the rapid detection of dermatophilus congolensis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885745/
https://www.ncbi.nlm.nih.gov/pubmed/23820221
http://dx.doi.org/10.4142/jvs.2013.14.4.491
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