6K(2)-induced vesicles can move cell to cell during turnip mosaic virus infection

To successfully infect plants, viruses replicate in an initially infected cell and then move to neighboring cells through plasmodesmata (PDs). However, the nature of the viral entity that crosses over the cell barrier into non-infected ones is not clear. The membrane-associated 6K(2) protein of turnip mosaic virus (TuMV) induces the formation of vesicles involved in the replication and intracellular movement of viral RNA. This study shows that 6K(2)-induced vesicles trafficked toward the plasma membrane and were associated with plasmodesmata (PD). We demonstrated also that 6K(2) moved cell-to-cell into adjoining cells when plants were infected with TuMV. 6K(2) was then fused to photo-activable GFP (6K(2):PAGFP) to visualize how 6K(2) moved intercellularly during TuMV infection. After activation, 6K(2):PAGFP-tagged vesicles moved to the cell periphery and across the cell wall into adjacent cells. These vesicles were shown to contain the viral RNA-dependent RNA polymerase and viral RNA. Symplasmic movement of TuMV may thus be achieved in the form of a membrane-associated viral RNA complex induced by 6K(2).