Background free imaging of single mRNAs in live cells using split fluorescent proteins

We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP t...

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Detalles Bibliográficos
Autores principales: Wu, Bin, Chen, Jiahao, Singer, Robert H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885884/
https://www.ncbi.nlm.nih.gov/pubmed/24402470
http://dx.doi.org/10.1038/srep03615
Descripción
Sumario:We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.

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