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Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography
OBJECTIVES: The aim of this study is to compare two commercially available screw-type sandblasted and acid-etched (SLA) Ti implant systems from Eckermann Laboratorium S.L., with similar geometry and distinct microtopography, regarding surface properties and osteoblastic cytocompatibility. MATERIAL A...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Stilus Optimus
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886090/ https://www.ncbi.nlm.nih.gov/pubmed/24422006 http://dx.doi.org/10.5037/jomr.2012.3104 |
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author | Mesquita, Pedro Gomes, Pedro de Sousa Sampaio, Paula Juodzbalys, Gintaras Afonso, Américo Fernandes, Maria Helena |
author_facet | Mesquita, Pedro Gomes, Pedro de Sousa Sampaio, Paula Juodzbalys, Gintaras Afonso, Américo Fernandes, Maria Helena |
author_sort | Mesquita, Pedro |
collection | PubMed |
description | OBJECTIVES: The aim of this study is to compare two commercially available screw-type sandblasted and acid-etched (SLA) Ti implant systems from Eckermann Laboratorium S.L., with similar geometry and distinct microtopography, regarding surface properties and osteoblastic cytocompatibility. MATERIAL AND METHODS: Implant I (referred as a conventional SLA system) and Implant II (a system patented as Eckcyte(®)) were characterized for macro and microtopograpphy, surface roughness and chemical composition. For the cytocompatibility studies, human bone marrow osteoblastic cells were seeded over the implants' surface, and the cell response was assessed for cell adhesion and proliferation, alkaline phosphatase (ALP) activity and matrix mineralization. RESULTS: Implant I presented a rough surface with irregularly shaped and sized cavities among flatter-appearing areas, whereas Implant II exhibited a homogeneous rough microporous surface. Compared to Implant I, Implant II presented higher Ra values (0.8 [SD 0.008] μm and 1.21 [SD 0.15] μm, respectively, P < 0.05) and also increased values of Rz, Rt and Rsm, a more negative value of Rsk, and similar RKu values. XPS showed the expected presence of Ti, O, C and N; Al, Si, F, P and Ca were detected in low concentrations. Implant II exhibited significantly lower Al levels. Both implants supported the adhesion, proliferation and differentiation of osteoblastic cells. Implant II showed a thicker fibrilar cell layer and an earlier onset and more abundant matrix mineralization. CONCLUSIONS: The homogeneous rough and microporous surface of Implant II is most probably a main contributor for its improved cell response. |
format | Online Article Text |
id | pubmed-3886090 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Stilus Optimus |
record_format | MEDLINE/PubMed |
spelling | pubmed-38860902014-01-13 Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography Mesquita, Pedro Gomes, Pedro de Sousa Sampaio, Paula Juodzbalys, Gintaras Afonso, Américo Fernandes, Maria Helena J Oral Maxillofac Res Original Paper OBJECTIVES: The aim of this study is to compare two commercially available screw-type sandblasted and acid-etched (SLA) Ti implant systems from Eckermann Laboratorium S.L., with similar geometry and distinct microtopography, regarding surface properties and osteoblastic cytocompatibility. MATERIAL AND METHODS: Implant I (referred as a conventional SLA system) and Implant II (a system patented as Eckcyte(®)) were characterized for macro and microtopograpphy, surface roughness and chemical composition. For the cytocompatibility studies, human bone marrow osteoblastic cells were seeded over the implants' surface, and the cell response was assessed for cell adhesion and proliferation, alkaline phosphatase (ALP) activity and matrix mineralization. RESULTS: Implant I presented a rough surface with irregularly shaped and sized cavities among flatter-appearing areas, whereas Implant II exhibited a homogeneous rough microporous surface. Compared to Implant I, Implant II presented higher Ra values (0.8 [SD 0.008] μm and 1.21 [SD 0.15] μm, respectively, P < 0.05) and also increased values of Rz, Rt and Rsm, a more negative value of Rsk, and similar RKu values. XPS showed the expected presence of Ti, O, C and N; Al, Si, F, P and Ca were detected in low concentrations. Implant II exhibited significantly lower Al levels. Both implants supported the adhesion, proliferation and differentiation of osteoblastic cells. Implant II showed a thicker fibrilar cell layer and an earlier onset and more abundant matrix mineralization. CONCLUSIONS: The homogeneous rough and microporous surface of Implant II is most probably a main contributor for its improved cell response. Stilus Optimus 2012-04-01 /pmc/articles/PMC3886090/ /pubmed/24422006 http://dx.doi.org/10.5037/jomr.2012.3104 Text en Copyright © Mesquita P, Gomes PD, Sampaio P, Juodzbalys G, Afonso A, Fernandes MH. Published in the JOURNAL OF ORAL & MAXILLOFACIAL RESEARCH (http://www.ejomr.org), 1 April 2012. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article, first published in the JOURNAL OF ORAL & MAXILLOFACIAL RESEARCH, distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work and is properly cited. The copyright, license information and link to the original publication on http://www.ejomr.org must be included. |
spellingShingle | Original Paper Mesquita, Pedro Gomes, Pedro de Sousa Sampaio, Paula Juodzbalys, Gintaras Afonso, Américo Fernandes, Maria Helena Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography |
title | Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography |
title_full | Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography |
title_fullStr | Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography |
title_full_unstemmed | Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography |
title_short | Surface Properties and Osteoblastic Cytocompatibility of Two Blasted and Acid-Etched Titanium Implant Systems with Distinct Microtopography |
title_sort | surface properties and osteoblastic cytocompatibility of two blasted and acid-etched titanium implant systems with distinct microtopography |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886090/ https://www.ncbi.nlm.nih.gov/pubmed/24422006 http://dx.doi.org/10.5037/jomr.2012.3104 |
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