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Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard
Rapid diagnostic tests are needed for typhoid fever (TF) diagnosis in febrile children in endemic areas. Five hundred children admitted to the hospital in Cambodia between 2009 and 2010 with documented fever (≥ 38°C) were investigated using blood cultures (BCs), Salmonella Typhi/Paratyphi A real-tim...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society of Tropical Medicine and Hygiene
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886406/ https://www.ncbi.nlm.nih.gov/pubmed/24218407 http://dx.doi.org/10.4269/ajtmh.13-0384 |
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author | Moore, Catrin E. Pan-Ngum, Wirichada Wijedoru, Lalith P. M. Sona, Soeng Nga, Tran Vu Thieu Duy, Pham Thanh Vinh, Phat Voong Chheng, Kheng Kumar, Varun Emary, Kate Carter, Michael White, Lisa Baker, Stephen Day, Nicholas P. J. Parry, Christopher M. |
author_facet | Moore, Catrin E. Pan-Ngum, Wirichada Wijedoru, Lalith P. M. Sona, Soeng Nga, Tran Vu Thieu Duy, Pham Thanh Vinh, Phat Voong Chheng, Kheng Kumar, Varun Emary, Kate Carter, Michael White, Lisa Baker, Stephen Day, Nicholas P. J. Parry, Christopher M. |
author_sort | Moore, Catrin E. |
collection | PubMed |
description | Rapid diagnostic tests are needed for typhoid fever (TF) diagnosis in febrile children in endemic areas. Five hundred children admitted to the hospital in Cambodia between 2009 and 2010 with documented fever (≥ 38°C) were investigated using blood cultures (BCs), Salmonella Typhi/Paratyphi A real-time polymerase chain reactions (PCRs), and a Typhoid immunoglobulin M flow assay (IgMFA). Test performance was determined by conventional methods and Bayesian latent class modeling. There were 32 cases of TF (10 BC- and PCR-positive cases, 14 BC-positive and PCR-negative cases, and 8 BC-negative and PCR-positive cases). IgMFA sensitivity was 59.4% (95% confidence interval = 41–76), and specificity was 97.8% (95% confidence interval = 96–99). The model estimate sensitivity for BC was 81.0% (95% credible interval = 54–99). The model estimate sensitivity for PCR was 37.8% (95% credible interval = 26–55), with a specificity of 98.2% (95% credible interval = 97–99). The model estimate sensitivity for IgMFA (≥ 2+) was 77.9% (95% credible interval = 58–90), with a specificity of 97.5% (95% credible interval = 95–100). The model estimates of IgMFA sensitivity and specificity were comparable with BCs and better than estimates using conventional analysis. |
format | Online Article Text |
id | pubmed-3886406 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The American Society of Tropical Medicine and Hygiene |
record_format | MEDLINE/PubMed |
spelling | pubmed-38864062014-01-10 Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard Moore, Catrin E. Pan-Ngum, Wirichada Wijedoru, Lalith P. M. Sona, Soeng Nga, Tran Vu Thieu Duy, Pham Thanh Vinh, Phat Voong Chheng, Kheng Kumar, Varun Emary, Kate Carter, Michael White, Lisa Baker, Stephen Day, Nicholas P. J. Parry, Christopher M. Am J Trop Med Hyg Articles Rapid diagnostic tests are needed for typhoid fever (TF) diagnosis in febrile children in endemic areas. Five hundred children admitted to the hospital in Cambodia between 2009 and 2010 with documented fever (≥ 38°C) were investigated using blood cultures (BCs), Salmonella Typhi/Paratyphi A real-time polymerase chain reactions (PCRs), and a Typhoid immunoglobulin M flow assay (IgMFA). Test performance was determined by conventional methods and Bayesian latent class modeling. There were 32 cases of TF (10 BC- and PCR-positive cases, 14 BC-positive and PCR-negative cases, and 8 BC-negative and PCR-positive cases). IgMFA sensitivity was 59.4% (95% confidence interval = 41–76), and specificity was 97.8% (95% confidence interval = 96–99). The model estimate sensitivity for BC was 81.0% (95% credible interval = 54–99). The model estimate sensitivity for PCR was 37.8% (95% credible interval = 26–55), with a specificity of 98.2% (95% credible interval = 97–99). The model estimate sensitivity for IgMFA (≥ 2+) was 77.9% (95% credible interval = 58–90), with a specificity of 97.5% (95% credible interval = 95–100). The model estimates of IgMFA sensitivity and specificity were comparable with BCs and better than estimates using conventional analysis. The American Society of Tropical Medicine and Hygiene 2014-01-08 /pmc/articles/PMC3886406/ /pubmed/24218407 http://dx.doi.org/10.4269/ajtmh.13-0384 Text en ©The American Society of Tropical Medicine and Hygiene This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Moore, Catrin E. Pan-Ngum, Wirichada Wijedoru, Lalith P. M. Sona, Soeng Nga, Tran Vu Thieu Duy, Pham Thanh Vinh, Phat Voong Chheng, Kheng Kumar, Varun Emary, Kate Carter, Michael White, Lisa Baker, Stephen Day, Nicholas P. J. Parry, Christopher M. Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard |
title | Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard |
title_full | Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard |
title_fullStr | Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard |
title_full_unstemmed | Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard |
title_short | Evaluation of the Diagnostic Accuracy of a Typhoid IgM Flow Assay for the Diagnosis of Typhoid Fever in Cambodian Children Using a Bayesian Latent Class Model Assuming an Imperfect Gold Standard |
title_sort | evaluation of the diagnostic accuracy of a typhoid igm flow assay for the diagnosis of typhoid fever in cambodian children using a bayesian latent class model assuming an imperfect gold standard |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886406/ https://www.ncbi.nlm.nih.gov/pubmed/24218407 http://dx.doi.org/10.4269/ajtmh.13-0384 |
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