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Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography

The electrochemical reduction of zanosar was investigated systematically by direct current polarography, cyclic voltammetry, and differential pulse polarography (DPP). A simple DPP technique was proposed for the direct quantitative determination of anticancer drug zanosar in pharmaceutical formulati...

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Autores principales: Reddy, Chennupalle Nageswara, ReddyPrasad, Puthalapattu, Sreedhar, NeelamYughandhar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886556/
https://www.ncbi.nlm.nih.gov/pubmed/24455423
http://dx.doi.org/10.1155/2013/420761
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author Reddy, Chennupalle Nageswara
ReddyPrasad, Puthalapattu
Sreedhar, NeelamYughandhar
author_facet Reddy, Chennupalle Nageswara
ReddyPrasad, Puthalapattu
Sreedhar, NeelamYughandhar
author_sort Reddy, Chennupalle Nageswara
collection PubMed
description The electrochemical reduction of zanosar was investigated systematically by direct current polarography, cyclic voltammetry, and differential pulse polarography (DPP). A simple DPP technique was proposed for the direct quantitative determination of anticancer drug zanosar in pharmaceutical formulation and spiked human urine samples for the first time. The reduction potential was −0.28 V versus Ag/AgCl with a hanging mercury drop electrode in Britton-Robinson buffer as supporting electrolyte. The dependence of the intensities of currents and potentials on pH, concentration, scan rate, deposition time, and nature of the supporting electrolyte was investigated. The calibration curve was found to be linear with the following equation: y = 0.4041x + 0.012, with a correlation coefficient of 0.992 (R (2)) over a concentration range from 1.0 × 10(−7) M to 1.0 × 10(−3) M. In the present investigation, the achieved limit of detection (LOD) and limit of quantization (LQD) were 7.42 × 10(−8) M and 2.47 × 10(−8) M; respectively. Excipients did not interfere with the determination of zanosar in pharmaceutical formulation and spiked urine samples. Precision and accuracy of the developed method were checked by recovery studies in pharmaceutical formulation and spiked human urine samples.
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spelling pubmed-38865562014-01-22 Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography Reddy, Chennupalle Nageswara ReddyPrasad, Puthalapattu Sreedhar, NeelamYughandhar J Anal Methods Chem Research Article The electrochemical reduction of zanosar was investigated systematically by direct current polarography, cyclic voltammetry, and differential pulse polarography (DPP). A simple DPP technique was proposed for the direct quantitative determination of anticancer drug zanosar in pharmaceutical formulation and spiked human urine samples for the first time. The reduction potential was −0.28 V versus Ag/AgCl with a hanging mercury drop electrode in Britton-Robinson buffer as supporting electrolyte. The dependence of the intensities of currents and potentials on pH, concentration, scan rate, deposition time, and nature of the supporting electrolyte was investigated. The calibration curve was found to be linear with the following equation: y = 0.4041x + 0.012, with a correlation coefficient of 0.992 (R (2)) over a concentration range from 1.0 × 10(−7) M to 1.0 × 10(−3) M. In the present investigation, the achieved limit of detection (LOD) and limit of quantization (LQD) were 7.42 × 10(−8) M and 2.47 × 10(−8) M; respectively. Excipients did not interfere with the determination of zanosar in pharmaceutical formulation and spiked urine samples. Precision and accuracy of the developed method were checked by recovery studies in pharmaceutical formulation and spiked human urine samples. Hindawi Publishing Corporation 2013 2013-12-24 /pmc/articles/PMC3886556/ /pubmed/24455423 http://dx.doi.org/10.1155/2013/420761 Text en Copyright © 2013 Chennupalle Nageswara Reddy et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Reddy, Chennupalle Nageswara
ReddyPrasad, Puthalapattu
Sreedhar, NeelamYughandhar
Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography
title Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography
title_full Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography
title_fullStr Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography
title_full_unstemmed Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography
title_short Electrochemical Analysis of Antichemotherapeutic Drug Zanosar in Pharmaceutical and Biological Samples by Differential Pulse Polarography
title_sort electrochemical analysis of antichemotherapeutic drug zanosar in pharmaceutical and biological samples by differential pulse polarography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886556/
https://www.ncbi.nlm.nih.gov/pubmed/24455423
http://dx.doi.org/10.1155/2013/420761
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