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The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack

Mature mammals exhibit very limited capacity for regeneration of auditory hair cells, while all non-mammalian vertebrates examined can regenerate them. In an effort to find therapeutic targets for deafness and balance disorders, scientists have examined gene expression patterns in auditory tissues u...

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Detalles Bibliográficos
Autores principales: Smith, Michael E., Rajadinakaran, Gopinath
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886832/
https://www.ncbi.nlm.nih.gov/pubmed/24416530
http://dx.doi.org/10.3390/microarrays2030186
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author Smith, Michael E.
Rajadinakaran, Gopinath
author_facet Smith, Michael E.
Rajadinakaran, Gopinath
author_sort Smith, Michael E.
collection PubMed
description Mature mammals exhibit very limited capacity for regeneration of auditory hair cells, while all non-mammalian vertebrates examined can regenerate them. In an effort to find therapeutic targets for deafness and balance disorders, scientists have examined gene expression patterns in auditory tissues under different developmental and experimental conditions. Microarray technology has allowed the large-scale study of gene expression profiles (transcriptomics) at whole-genome levels, but since mRNA expression does not necessarily correlate with protein expression, other methods, such as microRNA analysis and proteomics, are needed to better understand the process of hair cell regeneration. These technologies and some of the results of them are discussed in this review. Although there is a considerable amount of variability found between studies owing to different species, tissues and treatments, there is some concordance between cellular pathways important for hair cell regeneration. Since gene expression and proteomics data is now commonly submitted to centralized online databases, meta-analyses of these data may provide a better picture of pathways that are common to the process of hair cell regeneration and lead to potential therapeutics. Indeed, some of the proteins found to be regulated in the inner ear of animal models (e.g., IGF-1) have now gone through human clinical trials.
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spelling pubmed-38868322014-01-09 The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack Smith, Michael E. Rajadinakaran, Gopinath Microarrays (Basel) Review Mature mammals exhibit very limited capacity for regeneration of auditory hair cells, while all non-mammalian vertebrates examined can regenerate them. In an effort to find therapeutic targets for deafness and balance disorders, scientists have examined gene expression patterns in auditory tissues under different developmental and experimental conditions. Microarray technology has allowed the large-scale study of gene expression profiles (transcriptomics) at whole-genome levels, but since mRNA expression does not necessarily correlate with protein expression, other methods, such as microRNA analysis and proteomics, are needed to better understand the process of hair cell regeneration. These technologies and some of the results of them are discussed in this review. Although there is a considerable amount of variability found between studies owing to different species, tissues and treatments, there is some concordance between cellular pathways important for hair cell regeneration. Since gene expression and proteomics data is now commonly submitted to centralized online databases, meta-analyses of these data may provide a better picture of pathways that are common to the process of hair cell regeneration and lead to potential therapeutics. Indeed, some of the proteins found to be regulated in the inner ear of animal models (e.g., IGF-1) have now gone through human clinical trials. MDPI 2013-07-25 /pmc/articles/PMC3886832/ /pubmed/24416530 http://dx.doi.org/10.3390/microarrays2030186 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Smith, Michael E.
Rajadinakaran, Gopinath
The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack
title The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack
title_full The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack
title_fullStr The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack
title_full_unstemmed The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack
title_short The Transcriptomics to Proteomics of Hair Cell Regeneration: Looking for a Hair Cell in a Haystack
title_sort transcriptomics to proteomics of hair cell regeneration: looking for a hair cell in a haystack
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3886832/
https://www.ncbi.nlm.nih.gov/pubmed/24416530
http://dx.doi.org/10.3390/microarrays2030186
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