Stromal–Epithelial Crosstalk Provides a Suitable Microenvironment for the Progression of Ovarian Cancer Cells in Vitro

The tumor microenvironment plays an important role in the progression of cancer. This study focused on carcinoma-associated fibroblasts (CAFs) and stromal–epithelial interaction between CAFs and epithelial ovarian carcinoma (EOC) cells. We isolated and established primary cultures of CAFs and co-cul...

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Detalles Bibliográficos
Autores principales: Fu, Shilong, Dong, Lihua, Sun, Wei, Xu, Yi, Gao, Li, Miao, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Informa Healthcare 2013
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3887600/
https://www.ncbi.nlm.nih.gov/pubmed/24147897
http://dx.doi.org/10.3109/07357907.2013.849723
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author Fu, Shilong
Dong, Lihua
Sun, Wei
Xu, Yi
Gao, Li
Miao, Yi
author_facet Fu, Shilong
Dong, Lihua
Sun, Wei
Xu, Yi
Gao, Li
Miao, Yi
author_sort Fu, Shilong
collection PubMed
description The tumor microenvironment plays an important role in the progression of cancer. This study focused on carcinoma-associated fibroblasts (CAFs) and stromal–epithelial interaction between CAFs and epithelial ovarian carcinoma (EOC) cells. We isolated and established primary cultures of CAFs and co-cultured CAFs and EOC cells in vitro. The co-culture conditioned medium (CC-CM) was harvested and its influence on EOC cells was examined. Cytokine, chemokine, and growth factor levels were screened using a biotin label-based human antibody array system. We found that the stromal–epithelial crosstalk provided a suitable microenvironment for the progression of ovarian cancer cells in vitro.
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spelling pubmed-38876002014-01-13 Stromal–Epithelial Crosstalk Provides a Suitable Microenvironment for the Progression of Ovarian Cancer Cells in Vitro Fu, Shilong Dong, Lihua Sun, Wei Xu, Yi Gao, Li Miao, Yi Cancer Invest Original Article The tumor microenvironment plays an important role in the progression of cancer. This study focused on carcinoma-associated fibroblasts (CAFs) and stromal–epithelial interaction between CAFs and epithelial ovarian carcinoma (EOC) cells. We isolated and established primary cultures of CAFs and co-cultured CAFs and EOC cells in vitro. The co-culture conditioned medium (CC-CM) was harvested and its influence on EOC cells was examined. Cytokine, chemokine, and growth factor levels were screened using a biotin label-based human antibody array system. We found that the stromal–epithelial crosstalk provided a suitable microenvironment for the progression of ovarian cancer cells in vitro. Informa Healthcare 2013-11 2013-10-22 /pmc/articles/PMC3887600/ /pubmed/24147897 http://dx.doi.org/10.3109/07357907.2013.849723 Text en © 2013 Informa Healthcare USA, Inc. http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited.
spellingShingle Original Article
Fu, Shilong
Dong, Lihua
Sun, Wei
Xu, Yi
Gao, Li
Miao, Yi
Stromal–Epithelial Crosstalk Provides a Suitable Microenvironment for the Progression of Ovarian Cancer Cells in Vitro
title Stromal–Epithelial Crosstalk Provides a Suitable Microenvironment for the Progression of Ovarian Cancer Cells in Vitro
title_full Stromal–Epithelial Crosstalk Provides a Suitable Microenvironment for the Progression of Ovarian Cancer Cells in Vitro
title_fullStr Stromal–Epithelial Crosstalk Provides a Suitable Microenvironment for the Progression of Ovarian Cancer Cells in Vitro
title_full_unstemmed Stromal–Epithelial Crosstalk Provides a Suitable Microenvironment for the Progression of Ovarian Cancer Cells in Vitro
title_short Stromal–Epithelial Crosstalk Provides a Suitable Microenvironment for the Progression of Ovarian Cancer Cells in Vitro
title_sort stromal–epithelial crosstalk provides a suitable microenvironment for the progression of ovarian cancer cells in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3887600/
https://www.ncbi.nlm.nih.gov/pubmed/24147897
http://dx.doi.org/10.3109/07357907.2013.849723
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