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AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration
PURPOSE: To localize and identify the gene and mutations causing autosomal recessive retinal dystrophy in two consanguineous Pakistani families. METHODS: Consanguineous families from Pakistan were ascertained to be affected with autosomal recessive retinal degeneration. All affected individuals unde...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888496/ https://www.ncbi.nlm.nih.gov/pubmed/24426771 |
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author | Li, David Jin, Chongfei Jiao, Xiaodong Li, Lin Bushra, Tahmina Naeem, Muhammad Asif Butt, Nadeem H. Husnain, Tayyab Sieving, Paul A. Riazuddin, Sheikh Riazuddin, S. Amer Hejtmancik, J. Fielding |
author_facet | Li, David Jin, Chongfei Jiao, Xiaodong Li, Lin Bushra, Tahmina Naeem, Muhammad Asif Butt, Nadeem H. Husnain, Tayyab Sieving, Paul A. Riazuddin, Sheikh Riazuddin, S. Amer Hejtmancik, J. Fielding |
author_sort | Li, David |
collection | PubMed |
description | PURPOSE: To localize and identify the gene and mutations causing autosomal recessive retinal dystrophy in two consanguineous Pakistani families. METHODS: Consanguineous families from Pakistan were ascertained to be affected with autosomal recessive retinal degeneration. All affected individuals underwent thorough ophthalmologic examinations. Blood samples were collected, and genomic DNA was extracted using a salting out procedure. Genotyping was performed using microsatellite markers spaced at approximately 10 cM intervals. Two-point linkage analysis was performed with the lod score method. Direct DNA sequencing of amplified genomic DNA was performed for mutation screening of candidate genes. RESULTS: Genome-wide linkage scans yielded a lod score of 3.05 at θ=0 for D17S1832 and 3.82 at θ=0 for D17S938, localizing the disease gene to a 12.22 cM (6.64 Mb) region flanked by D17S1828 and D17S1852 for family 61032 and family 61227, which contains aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), a gene previously implicated in recessive Leber congenital amaurosis and autosomal dominant cone-rod dystrophy. Sequencing of AIPL1 showed a homozygous c.773G>C (p.Arg258Pro) sequence change in all affected individuals of family 61032 and a homozygous c.465G>T (p.(H93_Q155del)) change in all affected members of family 61227. CONCLUSIONS: The results strongly suggest that the c.773G>C (p.R258P) and c.465G>T (p.(H93_Q155del)) mutations in AIPL1 cause autosomal recessive retinal degeneration in these consanguineous Pakistani families. |
format | Online Article Text |
id | pubmed-3888496 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-38884962014-01-14 AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration Li, David Jin, Chongfei Jiao, Xiaodong Li, Lin Bushra, Tahmina Naeem, Muhammad Asif Butt, Nadeem H. Husnain, Tayyab Sieving, Paul A. Riazuddin, Sheikh Riazuddin, S. Amer Hejtmancik, J. Fielding Mol Vis Research Article PURPOSE: To localize and identify the gene and mutations causing autosomal recessive retinal dystrophy in two consanguineous Pakistani families. METHODS: Consanguineous families from Pakistan were ascertained to be affected with autosomal recessive retinal degeneration. All affected individuals underwent thorough ophthalmologic examinations. Blood samples were collected, and genomic DNA was extracted using a salting out procedure. Genotyping was performed using microsatellite markers spaced at approximately 10 cM intervals. Two-point linkage analysis was performed with the lod score method. Direct DNA sequencing of amplified genomic DNA was performed for mutation screening of candidate genes. RESULTS: Genome-wide linkage scans yielded a lod score of 3.05 at θ=0 for D17S1832 and 3.82 at θ=0 for D17S938, localizing the disease gene to a 12.22 cM (6.64 Mb) region flanked by D17S1828 and D17S1852 for family 61032 and family 61227, which contains aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), a gene previously implicated in recessive Leber congenital amaurosis and autosomal dominant cone-rod dystrophy. Sequencing of AIPL1 showed a homozygous c.773G>C (p.Arg258Pro) sequence change in all affected individuals of family 61032 and a homozygous c.465G>T (p.(H93_Q155del)) change in all affected members of family 61227. CONCLUSIONS: The results strongly suggest that the c.773G>C (p.R258P) and c.465G>T (p.(H93_Q155del)) mutations in AIPL1 cause autosomal recessive retinal degeneration in these consanguineous Pakistani families. Molecular Vision 2014-01-06 /pmc/articles/PMC3888496/ /pubmed/24426771 Text en Copyright © 2014 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Li, David Jin, Chongfei Jiao, Xiaodong Li, Lin Bushra, Tahmina Naeem, Muhammad Asif Butt, Nadeem H. Husnain, Tayyab Sieving, Paul A. Riazuddin, Sheikh Riazuddin, S. Amer Hejtmancik, J. Fielding AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration |
title | AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration |
title_full | AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration |
title_fullStr | AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration |
title_full_unstemmed | AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration |
title_short | AIPL1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration |
title_sort | aipl1 implicated in the pathogenesis of two cases of autosomal recessive retinal degeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888496/ https://www.ncbi.nlm.nih.gov/pubmed/24426771 |
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