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Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration

Titanium was treated with 3,4-dihydroxy-L-phenylalanine (DOPA) or dopamine to immobilize bone morphogenetic protein-2 (BMP2), a biomolecule. DOPA and dopamine solutions turned into suspensions, and precipitates were produced at high pH. Both treatments produced a brown surface on titanium that was t...

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Detalles Bibliográficos
Autores principales: Kang, Jeonghwa, Tada, Seiichi, Kitajima, Takashi, Son, Tae Il, Aigaki, Toshiro, Ito, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888698/
https://www.ncbi.nlm.nih.gov/pubmed/24459666
http://dx.doi.org/10.1155/2013/265980
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author Kang, Jeonghwa
Tada, Seiichi
Kitajima, Takashi
Son, Tae Il
Aigaki, Toshiro
Ito, Yoshihiro
author_facet Kang, Jeonghwa
Tada, Seiichi
Kitajima, Takashi
Son, Tae Il
Aigaki, Toshiro
Ito, Yoshihiro
author_sort Kang, Jeonghwa
collection PubMed
description Titanium was treated with 3,4-dihydroxy-L-phenylalanine (DOPA) or dopamine to immobilize bone morphogenetic protein-2 (BMP2), a biomolecule. DOPA and dopamine solutions turned into suspensions, and precipitates were produced at high pH. Both treatments produced a brown surface on titanium that was thicker at high pH than low pH. Dopamine produced a thicker layer than DOPA. The hydrophobicity of the surfaces increased after treatment with dopamine independent of pH. Furthermore, there were more amino groups in the layers formed at pH 8.5 than pH 4.5 in both treatments. Dopamine treatment produced more amino groups in the layer than DOPA. BMP2 was immobilized on the treated surfaces via a coupling reaction using carbodiimide. More BMP2 was immobilized on surfaces treated at pH 8.5 than pH 4.5 in both treatments. The immobilized BMP induced specific signal transduction and alkali phosphatase, a differentiation marker. Thus, the present study demonstrates that titanium treated with DOPA or dopamine can become bioactive via the surface immobilization of BMP2, which induces specific signal transduction.
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spelling pubmed-38886982014-01-23 Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration Kang, Jeonghwa Tada, Seiichi Kitajima, Takashi Son, Tae Il Aigaki, Toshiro Ito, Yoshihiro Biomed Res Int Research Article Titanium was treated with 3,4-dihydroxy-L-phenylalanine (DOPA) or dopamine to immobilize bone morphogenetic protein-2 (BMP2), a biomolecule. DOPA and dopamine solutions turned into suspensions, and precipitates were produced at high pH. Both treatments produced a brown surface on titanium that was thicker at high pH than low pH. Dopamine produced a thicker layer than DOPA. The hydrophobicity of the surfaces increased after treatment with dopamine independent of pH. Furthermore, there were more amino groups in the layers formed at pH 8.5 than pH 4.5 in both treatments. Dopamine treatment produced more amino groups in the layer than DOPA. BMP2 was immobilized on the treated surfaces via a coupling reaction using carbodiimide. More BMP2 was immobilized on surfaces treated at pH 8.5 than pH 4.5 in both treatments. The immobilized BMP induced specific signal transduction and alkali phosphatase, a differentiation marker. Thus, the present study demonstrates that titanium treated with DOPA or dopamine can become bioactive via the surface immobilization of BMP2, which induces specific signal transduction. Hindawi Publishing Corporation 2013 2013-12-28 /pmc/articles/PMC3888698/ /pubmed/24459666 http://dx.doi.org/10.1155/2013/265980 Text en Copyright © 2013 Jeonghwa Kang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kang, Jeonghwa
Tada, Seiichi
Kitajima, Takashi
Son, Tae Il
Aigaki, Toshiro
Ito, Yoshihiro
Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration
title Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration
title_full Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration
title_fullStr Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration
title_full_unstemmed Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration
title_short Immobilization of Bone Morphogenetic Protein on DOPA- or Dopamine-Treated Titanium Surfaces to Enhance Osseointegration
title_sort immobilization of bone morphogenetic protein on dopa- or dopamine-treated titanium surfaces to enhance osseointegration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888698/
https://www.ncbi.nlm.nih.gov/pubmed/24459666
http://dx.doi.org/10.1155/2013/265980
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