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Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model

Background. The aim of this study was to evaluate the use of liquid perfluorocarbon (PFC) as an adjuvant substance for lung preservation and assess its role in pulmonary protection after transplantation. Methods. Seventy-two rat lungs were flushed with low-potassium dextran (LPD) solution and random...

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Autores principales: Forgiarini Junior, Luiz Alberto, Holand, Arthur Rodrigo Ronconi, Forgiarini, Luiz Felipe, da Rosa, Darlan Pase, Marroni, Norma Anair Possa, Cardoso, Paulo Francisco Guerreiro, Andrade, Cristiano Feijó
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888767/
https://www.ncbi.nlm.nih.gov/pubmed/24453412
http://dx.doi.org/10.1155/2013/193484
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author Forgiarini Junior, Luiz Alberto
Holand, Arthur Rodrigo Ronconi
Forgiarini, Luiz Felipe
da Rosa, Darlan Pase
Marroni, Norma Anair Possa
Cardoso, Paulo Francisco Guerreiro
Andrade, Cristiano Feijó
author_facet Forgiarini Junior, Luiz Alberto
Holand, Arthur Rodrigo Ronconi
Forgiarini, Luiz Felipe
da Rosa, Darlan Pase
Marroni, Norma Anair Possa
Cardoso, Paulo Francisco Guerreiro
Andrade, Cristiano Feijó
author_sort Forgiarini Junior, Luiz Alberto
collection PubMed
description Background. The aim of this study was to evaluate the use of liquid perfluorocarbon (PFC) as an adjuvant substance for lung preservation and assess its role in pulmonary protection after transplantation. Methods. Seventy-two rat lungs were flushed with low-potassium dextran (LPD) solution and randomized into three main groups: control with LPD alone and experimental with 3 (PFC3) and 7 mL/kg (PFC7) of endobronchial PFC instilled just after harvest. Each group was divided into four subgroups according to preservation time (3, 6, 12, and 24 hours). Afterwards, we performed lung transplantation using rat lungs preserved for 12 hours with LPD alone or with 7 mL/kg of endobronchial PFC. Results. There was a significant increase in oxidative stress in the control group at 6 h of cold ischemic time compared with the PFC3 and PFC7 groups. The apoptotic activity and NF-κB expression were significantly higher in the control group compared with the PFC groups at 3, 12, and 24 h of cold preservation. After transplantation, the NF-κB, iNOS, and nitrotyrosine expression as well as caspase 3 activity were significantly lower in the PFC groups. Conclusion. The use of endobronchial PFC as an adjuvant to the current preservation strategy improved graft viability.
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spelling pubmed-38887672014-01-22 Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model Forgiarini Junior, Luiz Alberto Holand, Arthur Rodrigo Ronconi Forgiarini, Luiz Felipe da Rosa, Darlan Pase Marroni, Norma Anair Possa Cardoso, Paulo Francisco Guerreiro Andrade, Cristiano Feijó Mediators Inflamm Research Article Background. The aim of this study was to evaluate the use of liquid perfluorocarbon (PFC) as an adjuvant substance for lung preservation and assess its role in pulmonary protection after transplantation. Methods. Seventy-two rat lungs were flushed with low-potassium dextran (LPD) solution and randomized into three main groups: control with LPD alone and experimental with 3 (PFC3) and 7 mL/kg (PFC7) of endobronchial PFC instilled just after harvest. Each group was divided into four subgroups according to preservation time (3, 6, 12, and 24 hours). Afterwards, we performed lung transplantation using rat lungs preserved for 12 hours with LPD alone or with 7 mL/kg of endobronchial PFC. Results. There was a significant increase in oxidative stress in the control group at 6 h of cold ischemic time compared with the PFC3 and PFC7 groups. The apoptotic activity and NF-κB expression were significantly higher in the control group compared with the PFC groups at 3, 12, and 24 h of cold preservation. After transplantation, the NF-κB, iNOS, and nitrotyrosine expression as well as caspase 3 activity were significantly lower in the PFC groups. Conclusion. The use of endobronchial PFC as an adjuvant to the current preservation strategy improved graft viability. Hindawi Publishing Corporation 2013 2013-12-26 /pmc/articles/PMC3888767/ /pubmed/24453412 http://dx.doi.org/10.1155/2013/193484 Text en Copyright © 2013 Luiz Alberto Forgiarini Junior et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Forgiarini Junior, Luiz Alberto
Holand, Arthur Rodrigo Ronconi
Forgiarini, Luiz Felipe
da Rosa, Darlan Pase
Marroni, Norma Anair Possa
Cardoso, Paulo Francisco Guerreiro
Andrade, Cristiano Feijó
Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model
title Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model
title_full Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model
title_fullStr Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model
title_full_unstemmed Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model
title_short Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model
title_sort endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888767/
https://www.ncbi.nlm.nih.gov/pubmed/24453412
http://dx.doi.org/10.1155/2013/193484
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