Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum

We used SDS-polyacrylamide gel electrophoresis to investigate the outer membrane proteins (OMPs) band composition of 19 Escherichia coli K1 strains that have spontaneously lost the ability to form K1 polysaccharide capsule (E. coli K1−) and demonstrated different degrees of susceptibility to the bac...

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Autores principales: Cisowska, Agnieszka, Bugla-Płoskońska, Gabriela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3889503/
https://www.ncbi.nlm.nih.gov/pubmed/23794053
http://dx.doi.org/10.1007/s12223-013-0262-6
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author Cisowska, Agnieszka
Bugla-Płoskońska, Gabriela
author_facet Cisowska, Agnieszka
Bugla-Płoskońska, Gabriela
author_sort Cisowska, Agnieszka
collection PubMed
description We used SDS-polyacrylamide gel electrophoresis to investigate the outer membrane proteins (OMPs) band composition of 19 Escherichia coli K1 strains that have spontaneously lost the ability to form K1 polysaccharide capsule (E. coli K1−) and demonstrated different degrees of susceptibility to the bactericidal action of normal human serum. Presented results showed that there were differences between E. coli K1− strains in OMPs expressing capacity. The analysis performed on OMPs has not revealed a direct association between the different OMPs band composition and the susceptibility of these strains to the serum.
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spelling pubmed-38895032014-01-14 Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum Cisowska, Agnieszka Bugla-Płoskońska, Gabriela Folia Microbiol (Praha) Article We used SDS-polyacrylamide gel electrophoresis to investigate the outer membrane proteins (OMPs) band composition of 19 Escherichia coli K1 strains that have spontaneously lost the ability to form K1 polysaccharide capsule (E. coli K1−) and demonstrated different degrees of susceptibility to the bactericidal action of normal human serum. Presented results showed that there were differences between E. coli K1− strains in OMPs expressing capacity. The analysis performed on OMPs has not revealed a direct association between the different OMPs band composition and the susceptibility of these strains to the serum. Springer Netherlands 2013-06-22 2014 /pmc/articles/PMC3889503/ /pubmed/23794053 http://dx.doi.org/10.1007/s12223-013-0262-6 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Cisowska, Agnieszka
Bugla-Płoskońska, Gabriela
Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum
title Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum
title_full Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum
title_fullStr Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum
title_full_unstemmed Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum
title_short Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum
title_sort analysis of the sds-page patterns of outer membrane proteins from escherichia coli strains that have lost the ability to form k1 antigen and varied in the susceptibility to normal human serum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3889503/
https://www.ncbi.nlm.nih.gov/pubmed/23794053
http://dx.doi.org/10.1007/s12223-013-0262-6
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AT buglapłoskonskagabriela analysisofthesdspagepatternsofoutermembraneproteinsfromescherichiacolistrainsthathavelosttheabilitytoformk1antigenandvariedinthesusceptibilitytonormalhumanserum

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