Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase

We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This met...

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Detalles Bibliográficos
Autores principales: Ryder, Edward, Doe, Brendan, Gleeson, Diane, Houghton, Richard, Dalvi, Priya, Grau, Evelyn, Habib, Bishoy, Miklejewska, Evelina, Newman, Stuart, Sethi, Debarati, Sinclair, Caroline, Vyas, Sapna, Wardle-Jones, Hannah, Bottomley, Joanna, Bussell, James, Galli, Antonella, Salisbury, Jennifer, Ramirez-Solis, Ramiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890051/
https://www.ncbi.nlm.nih.gov/pubmed/24197666
http://dx.doi.org/10.1007/s11248-013-9764-x
Descripción
Sumario:We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11248-013-9764-x) contains supplementary material, which is available to authorized users.

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