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Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development

For correct functioning of the nervous system, the appropriate number and complement of neuronal cell types must be produced during development. However, the molecular mechanisms that regulate the production of individual classes of neurons are poorly understood. In this study, we investigate the fu...

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Autores principales: Nakamoto, Chizu, Kuan, Soh-Leh, Findlay, Amy S., Durward, Elaine, Ouyang, Zhufeng, Zakrzewska, Ewa D., Endo, Takuma, Nakamoto, Masaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890344/
https://www.ncbi.nlm.nih.gov/pubmed/24258025
http://dx.doi.org/10.1091/mbc.E13-08-0453
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author Nakamoto, Chizu
Kuan, Soh-Leh
Findlay, Amy S.
Durward, Elaine
Ouyang, Zhufeng
Zakrzewska, Ewa D.
Endo, Takuma
Nakamoto, Masaru
author_facet Nakamoto, Chizu
Kuan, Soh-Leh
Findlay, Amy S.
Durward, Elaine
Ouyang, Zhufeng
Zakrzewska, Ewa D.
Endo, Takuma
Nakamoto, Masaru
author_sort Nakamoto, Chizu
collection PubMed
description For correct functioning of the nervous system, the appropriate number and complement of neuronal cell types must be produced during development. However, the molecular mechanisms that regulate the production of individual classes of neurons are poorly understood. In this study, we investigate the function of the thrombospondin-1–like glycoprotein, Nel (neural epidermal growth factor [EGF]-like), in the generation of retinal ganglion cells (RGCs) in chicks. During eye development, Nel is strongly expressed in the presumptive retinal pigment epithelium and RGCs. Nel overexpression in the developing retina by in ovo electroporation increases the number of RGCs, whereas the number of displaced amacrine cells decreases. Conversely, knockdown of Nel expression by transposon-mediated introduction of RNA interference constructs results in decrease in RGC number and increase in the number of displaced amacrine cells. Modifications of Nel expression levels do not appear to affect proliferation of retinal progenitor cells, but they significantly alter the progression rate of RGC differentiation from the central retina to the periphery. Furthermore, Nel protects RGCs from apoptosis during retinal development. These results indicate that Nel positively regulates RGC production by promoting their differentiation and survival during development.
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spelling pubmed-38903442014-03-30 Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development Nakamoto, Chizu Kuan, Soh-Leh Findlay, Amy S. Durward, Elaine Ouyang, Zhufeng Zakrzewska, Ewa D. Endo, Takuma Nakamoto, Masaru Mol Biol Cell Articles For correct functioning of the nervous system, the appropriate number and complement of neuronal cell types must be produced during development. However, the molecular mechanisms that regulate the production of individual classes of neurons are poorly understood. In this study, we investigate the function of the thrombospondin-1–like glycoprotein, Nel (neural epidermal growth factor [EGF]-like), in the generation of retinal ganglion cells (RGCs) in chicks. During eye development, Nel is strongly expressed in the presumptive retinal pigment epithelium and RGCs. Nel overexpression in the developing retina by in ovo electroporation increases the number of RGCs, whereas the number of displaced amacrine cells decreases. Conversely, knockdown of Nel expression by transposon-mediated introduction of RNA interference constructs results in decrease in RGC number and increase in the number of displaced amacrine cells. Modifications of Nel expression levels do not appear to affect proliferation of retinal progenitor cells, but they significantly alter the progression rate of RGC differentiation from the central retina to the periphery. Furthermore, Nel protects RGCs from apoptosis during retinal development. These results indicate that Nel positively regulates RGC production by promoting their differentiation and survival during development. The American Society for Cell Biology 2014-01-15 /pmc/articles/PMC3890344/ /pubmed/24258025 http://dx.doi.org/10.1091/mbc.E13-08-0453 Text en © 2014 Nakamoto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Nakamoto, Chizu
Kuan, Soh-Leh
Findlay, Amy S.
Durward, Elaine
Ouyang, Zhufeng
Zakrzewska, Ewa D.
Endo, Takuma
Nakamoto, Masaru
Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development
title Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development
title_full Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development
title_fullStr Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development
title_full_unstemmed Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development
title_short Nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development
title_sort nel positively regulates the genesis of retinal ganglion cells by promoting their differentiation and survival during development
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890344/
https://www.ncbi.nlm.nih.gov/pubmed/24258025
http://dx.doi.org/10.1091/mbc.E13-08-0453
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