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A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum

BACKGROUND: Inorganic phosphate (Pi) is essential for plant growth, and phosphorus deficiency is a main limiting factor in plant development. Its acquisition is largely mediated by Pht1 transporters, a family of plasma membrane-located proteins. Chrysanthemum is one of the most important ornamental...

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Autores principales: Liu, Peng, Chen, Sumei, Song, Aiping, Zhao, Shuang, Fang, Weimin, Guan, Zhiyong, Liao, Yuan, Jiang, Jiafu, Chen, Fadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890489/
https://www.ncbi.nlm.nih.gov/pubmed/24411021
http://dx.doi.org/10.1186/1471-2229-14-18
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author Liu, Peng
Chen, Sumei
Song, Aiping
Zhao, Shuang
Fang, Weimin
Guan, Zhiyong
Liao, Yuan
Jiang, Jiafu
Chen, Fadi
author_facet Liu, Peng
Chen, Sumei
Song, Aiping
Zhao, Shuang
Fang, Weimin
Guan, Zhiyong
Liao, Yuan
Jiang, Jiafu
Chen, Fadi
author_sort Liu, Peng
collection PubMed
description BACKGROUND: Inorganic phosphate (Pi) is essential for plant growth, and phosphorus deficiency is a main limiting factor in plant development. Its acquisition is largely mediated by Pht1 transporters, a family of plasma membrane-located proteins. Chrysanthemum is one of the most important ornamental plants, its productivity is usually compromised when grown in phosphate deficient soils, but the study of phosphate transporters in chrysanthemum is limited. RESULTS: We described the isolation from chrysanthemum of a homolog of the Phosphate Transporter 1 (PT1) family. Its predicted product is a protein with 12 transmembrane domains, highly homologous with other high affinity plant Pi transporters. Real-time quantitative PCR analysis revealed that the gene was transcribed strongly in the root, weakly in the stem and below the level of detection in the leaf of chrysanthemum plants growing in either sufficient or deficient Pi conditions. Transcript abundance was greatly enhanced in Pi-starved roots. A complementation assay in yeast showed that CmPT1 partially compensated for the absence of phosphate transporter activity in yeast strain MB192. The estimated K( m ) of CmPT1 was 35.2 μM. Under both Pi sufficient and deficient conditions, transgenic plants constitutively expressing CmPT1 grew taller than the non-transformed wild type, produced a greater volume of roots, accumulated more biomass and took up more phosphate. CONCLUSIONS: CmPT1 encodes a typical, root-expressed, high affinity phosphate transporter, plays an important role in coping Pi deficiency of chrysanthemum plants.
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spelling pubmed-38904892014-01-15 A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum Liu, Peng Chen, Sumei Song, Aiping Zhao, Shuang Fang, Weimin Guan, Zhiyong Liao, Yuan Jiang, Jiafu Chen, Fadi BMC Plant Biol Research Article BACKGROUND: Inorganic phosphate (Pi) is essential for plant growth, and phosphorus deficiency is a main limiting factor in plant development. Its acquisition is largely mediated by Pht1 transporters, a family of plasma membrane-located proteins. Chrysanthemum is one of the most important ornamental plants, its productivity is usually compromised when grown in phosphate deficient soils, but the study of phosphate transporters in chrysanthemum is limited. RESULTS: We described the isolation from chrysanthemum of a homolog of the Phosphate Transporter 1 (PT1) family. Its predicted product is a protein with 12 transmembrane domains, highly homologous with other high affinity plant Pi transporters. Real-time quantitative PCR analysis revealed that the gene was transcribed strongly in the root, weakly in the stem and below the level of detection in the leaf of chrysanthemum plants growing in either sufficient or deficient Pi conditions. Transcript abundance was greatly enhanced in Pi-starved roots. A complementation assay in yeast showed that CmPT1 partially compensated for the absence of phosphate transporter activity in yeast strain MB192. The estimated K( m ) of CmPT1 was 35.2 μM. Under both Pi sufficient and deficient conditions, transgenic plants constitutively expressing CmPT1 grew taller than the non-transformed wild type, produced a greater volume of roots, accumulated more biomass and took up more phosphate. CONCLUSIONS: CmPT1 encodes a typical, root-expressed, high affinity phosphate transporter, plays an important role in coping Pi deficiency of chrysanthemum plants. BioMed Central 2014-01-10 /pmc/articles/PMC3890489/ /pubmed/24411021 http://dx.doi.org/10.1186/1471-2229-14-18 Text en Copyright © 2014 Liu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Peng
Chen, Sumei
Song, Aiping
Zhao, Shuang
Fang, Weimin
Guan, Zhiyong
Liao, Yuan
Jiang, Jiafu
Chen, Fadi
A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum
title A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum
title_full A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum
title_fullStr A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum
title_full_unstemmed A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum
title_short A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum
title_sort putative high affinity phosphate transporter, cmpt1, enhances tolerance to pi deficiency of chrysanthemum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890489/
https://www.ncbi.nlm.nih.gov/pubmed/24411021
http://dx.doi.org/10.1186/1471-2229-14-18
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