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Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China

BACKGROUND: To determine the prevalence of human enteroviruses (HEVs) among healthy children, their parents, and children with hand, foot, and mouth disease (HFMD). METHODS: We conducted a case–control study that included throat samples from 579 children with HFMD and from 254 healthy controls. Thro...

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Autores principales: Zhang, Xiaoai, Wang, Hongyu, Ding, Shujun, Wang, Xianjun, Chen, Xiaodan, Wo, Ying, Wang, Liyuan, Huang, Doudou, Liu, Wei, Cao, Wuchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890605/
https://www.ncbi.nlm.nih.gov/pubmed/24370001
http://dx.doi.org/10.1186/1471-2334-13-606
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author Zhang, Xiaoai
Wang, Hongyu
Ding, Shujun
Wang, Xianjun
Chen, Xiaodan
Wo, Ying
Wang, Liyuan
Huang, Doudou
Liu, Wei
Cao, Wuchun
author_facet Zhang, Xiaoai
Wang, Hongyu
Ding, Shujun
Wang, Xianjun
Chen, Xiaodan
Wo, Ying
Wang, Liyuan
Huang, Doudou
Liu, Wei
Cao, Wuchun
author_sort Zhang, Xiaoai
collection PubMed
description BACKGROUND: To determine the prevalence of human enteroviruses (HEVs) among healthy children, their parents, and children with hand, foot, and mouth disease (HFMD). METHODS: We conducted a case–control study that included throat samples from 579 children with HFMD and from 254 healthy controls. Throat samples from 49 households (98 parents and 53 healthy children) were also analyzed. Phylogenetic analysis was carried out to study genetic relationships of EV71 strains. RESULTS: The HEV positive rate in HFMD patients was significantly higher than that in healthy controls (76.0% vs. 23.2%, P < 0.001). The EV71 (43.7% vs. 15.0%, P < 0.001), CVA16 (18.0% vs. 2.8%, P < 0.001), and CVA10 (5.7% vs. 0.8%, P = 0.001) serotypes were significantly overrepresented in HFMD patients in comparison to healthy children. Other HEV serotypes were detected with comparable frequency in cases and controls. The HEV positive rate in severe HFMD patients was significantly higher than that in mild group (82.1% vs. 73.8%, P = 0.04). The EV71 (55.0% vs. 39.7%, P = 0.001) and CVA16 (11. 9% vs. 20.0%, P = 0.024) positive rate differed significantly between severe and mild HFMD patients. Other HEV serotypes were detected with comparable frequency between severe and mild HFMD patients. Among 49 households, 22 households (44.9%) had at least 1 family member positive for HEV. Children had significantly higher HEV positive rate than adult (28.3% vs. 14.3%, P = 0.037). The HEV positive rate was similar between mothers and fathers (12.24% vs. 16.32%, P = 0.56). The VP1 sequences of EV71 from HFMD patients and healthy children were nearly identical and all were clustered in the same clade, C4a. CONCLUSIONS: Our study demonstrated the co-circulation of multiple HEV serotypes in children with and without HFMD during epidemic. Our study deserves the attention on HFMD control.
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spelling pubmed-38906052014-01-15 Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China Zhang, Xiaoai Wang, Hongyu Ding, Shujun Wang, Xianjun Chen, Xiaodan Wo, Ying Wang, Liyuan Huang, Doudou Liu, Wei Cao, Wuchun BMC Infect Dis Research Article BACKGROUND: To determine the prevalence of human enteroviruses (HEVs) among healthy children, their parents, and children with hand, foot, and mouth disease (HFMD). METHODS: We conducted a case–control study that included throat samples from 579 children with HFMD and from 254 healthy controls. Throat samples from 49 households (98 parents and 53 healthy children) were also analyzed. Phylogenetic analysis was carried out to study genetic relationships of EV71 strains. RESULTS: The HEV positive rate in HFMD patients was significantly higher than that in healthy controls (76.0% vs. 23.2%, P < 0.001). The EV71 (43.7% vs. 15.0%, P < 0.001), CVA16 (18.0% vs. 2.8%, P < 0.001), and CVA10 (5.7% vs. 0.8%, P = 0.001) serotypes were significantly overrepresented in HFMD patients in comparison to healthy children. Other HEV serotypes were detected with comparable frequency in cases and controls. The HEV positive rate in severe HFMD patients was significantly higher than that in mild group (82.1% vs. 73.8%, P = 0.04). The EV71 (55.0% vs. 39.7%, P = 0.001) and CVA16 (11. 9% vs. 20.0%, P = 0.024) positive rate differed significantly between severe and mild HFMD patients. Other HEV serotypes were detected with comparable frequency between severe and mild HFMD patients. Among 49 households, 22 households (44.9%) had at least 1 family member positive for HEV. Children had significantly higher HEV positive rate than adult (28.3% vs. 14.3%, P = 0.037). The HEV positive rate was similar between mothers and fathers (12.24% vs. 16.32%, P = 0.56). The VP1 sequences of EV71 from HFMD patients and healthy children were nearly identical and all were clustered in the same clade, C4a. CONCLUSIONS: Our study demonstrated the co-circulation of multiple HEV serotypes in children with and without HFMD during epidemic. Our study deserves the attention on HFMD control. BioMed Central 2013-12-27 /pmc/articles/PMC3890605/ /pubmed/24370001 http://dx.doi.org/10.1186/1471-2334-13-606 Text en Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Xiaoai
Wang, Hongyu
Ding, Shujun
Wang, Xianjun
Chen, Xiaodan
Wo, Ying
Wang, Liyuan
Huang, Doudou
Liu, Wei
Cao, Wuchun
Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China
title Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China
title_full Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China
title_fullStr Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China
title_full_unstemmed Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China
title_short Prevalence of enteroviruses in children with and without hand, foot, and mouth disease in China
title_sort prevalence of enteroviruses in children with and without hand, foot, and mouth disease in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890605/
https://www.ncbi.nlm.nih.gov/pubmed/24370001
http://dx.doi.org/10.1186/1471-2334-13-606
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