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DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae)
Abstract. Phormia regina (the black fly) is a common Holarctic blow fly species which serves as a primary indicator taxon to estimate minimal post mortem intervals. It is also a major research model in physiological and neurological studies on insect feeding. Previous studies have shown a sequence d...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pensoft Publishers
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890676/ https://www.ncbi.nlm.nih.gov/pubmed/24453556 http://dx.doi.org/10.3897/zookeys.365.6202 |
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author | Jordaens, Kurt Sonet, Gontran Braet, Yves De Meyer, Marc Backeljau, Thierry Goovaerts, Frankie Bourguignon, Luc Desmyter, Stijn |
author_facet | Jordaens, Kurt Sonet, Gontran Braet, Yves De Meyer, Marc Backeljau, Thierry Goovaerts, Frankie Bourguignon, Luc Desmyter, Stijn |
author_sort | Jordaens, Kurt |
collection | PubMed |
description | Abstract. Phormia regina (the black fly) is a common Holarctic blow fly species which serves as a primary indicator taxon to estimate minimal post mortem intervals. It is also a major research model in physiological and neurological studies on insect feeding. Previous studies have shown a sequence divergence of up to 4.3% in the mitochondrial COI gene between W European and N American P. regina populations. Here, we DNA barcoded P. regina specimens from six N American and 17 W European populations and confirmed a mean sequence divergence of ca. 4% between the populations of the two continents, while sequence divergence within each continent was a ten-fold lower. Comparable mean mtDNA sequence divergences were observed for COII (3.7%) and cyt b (5.3%), but mean divergence was lower for 16S (0.4–0.6%). Intercontinental divergence at nuclear DNA was very low (≤ 0.1% for both 28S and ITS2), and we did not detect any morphological differentiation between N American and W European specimens. Therefore, we consider the strong differentiation at COI, COII and cyt b as intraspecific mtDNA sequence divergence that should be taken into account when using P. regina in forensic casework or experimental research. |
format | Online Article Text |
id | pubmed-3890676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-38906762014-01-16 DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae) Jordaens, Kurt Sonet, Gontran Braet, Yves De Meyer, Marc Backeljau, Thierry Goovaerts, Frankie Bourguignon, Luc Desmyter, Stijn Zookeys Article Abstract. Phormia regina (the black fly) is a common Holarctic blow fly species which serves as a primary indicator taxon to estimate minimal post mortem intervals. It is also a major research model in physiological and neurological studies on insect feeding. Previous studies have shown a sequence divergence of up to 4.3% in the mitochondrial COI gene between W European and N American P. regina populations. Here, we DNA barcoded P. regina specimens from six N American and 17 W European populations and confirmed a mean sequence divergence of ca. 4% between the populations of the two continents, while sequence divergence within each continent was a ten-fold lower. Comparable mean mtDNA sequence divergences were observed for COII (3.7%) and cyt b (5.3%), but mean divergence was lower for 16S (0.4–0.6%). Intercontinental divergence at nuclear DNA was very low (≤ 0.1% for both 28S and ITS2), and we did not detect any morphological differentiation between N American and W European specimens. Therefore, we consider the strong differentiation at COI, COII and cyt b as intraspecific mtDNA sequence divergence that should be taken into account when using P. regina in forensic casework or experimental research. Pensoft Publishers 2013-12-30 /pmc/articles/PMC3890676/ /pubmed/24453556 http://dx.doi.org/10.3897/zookeys.365.6202 Text en Kurt Jordaens, Gontran Sonet, Yves Braet, Marc De Meyer, Thierry Backeljau, Frankie Goovaerts, Luc Bourguignon, Stijn Desmyter http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Jordaens, Kurt Sonet, Gontran Braet, Yves De Meyer, Marc Backeljau, Thierry Goovaerts, Frankie Bourguignon, Luc Desmyter, Stijn DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae) |
title | DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae) |
title_full | DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae) |
title_fullStr | DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae) |
title_full_unstemmed | DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae) |
title_short | DNA barcoding and the differentiation between North American and West European Phormia regina (Diptera, Calliphoridae, Chrysomyinae) |
title_sort | dna barcoding and the differentiation between north american and west european phormia regina (diptera, calliphoridae, chrysomyinae) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890676/ https://www.ncbi.nlm.nih.gov/pubmed/24453556 http://dx.doi.org/10.3897/zookeys.365.6202 |
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