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Revisiting species delimitation within the genus Oxystele using DNA barcoding approach
Abstract. The genus Oxystele, a member of the highly diverse marine gastropod superfamily Trochoidea, is endemic to southern Africa. Members of the genus include some of the most abundant molluscs on southern African shores and are important components of littoral biodiversity in rocky intertidal ha...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pensoft Publishers
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890686/ https://www.ncbi.nlm.nih.gov/pubmed/24453566 http://dx.doi.org/10.3897/zookeys.365.5356 |
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author | Van Der Bank, Herman Herbert, Dai Greenfield, Richard Yessoufou, Kowiyou |
author_facet | Van Der Bank, Herman Herbert, Dai Greenfield, Richard Yessoufou, Kowiyou |
author_sort | Van Der Bank, Herman |
collection | PubMed |
description | Abstract. The genus Oxystele, a member of the highly diverse marine gastropod superfamily Trochoidea, is endemic to southern Africa. Members of the genus include some of the most abundant molluscs on southern African shores and are important components of littoral biodiversity in rocky intertidal habitats. Species delimitation within the genus is still controversial, especially regarding the complex O. impervia / O. variegata. Here, we assessed species boundaries within the genus using DNA barcoding and phylogenetic tree reconstruction. We analysed 56 specimens using the mitochondrial gene COI. Our analysis delimits five molecular operational taxonomic units (MOTUs), and distinguishes O. impervia from O. variegata. However, we reveal important discrepancies between MOTUs and morphology-based species identification and discuss alternative hypotheses that can account for this. Finally, we indicate the need for future study that includes additional genes, and the combination of both morphology and genetic techniques (e.g. AFLP or microsatellites) to get deeper insight into species delimitation within the genus. |
format | Online Article Text |
id | pubmed-3890686 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Pensoft Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-38906862014-01-16 Revisiting species delimitation within the genus Oxystele using DNA barcoding approach Van Der Bank, Herman Herbert, Dai Greenfield, Richard Yessoufou, Kowiyou Zookeys Article Abstract. The genus Oxystele, a member of the highly diverse marine gastropod superfamily Trochoidea, is endemic to southern Africa. Members of the genus include some of the most abundant molluscs on southern African shores and are important components of littoral biodiversity in rocky intertidal habitats. Species delimitation within the genus is still controversial, especially regarding the complex O. impervia / O. variegata. Here, we assessed species boundaries within the genus using DNA barcoding and phylogenetic tree reconstruction. We analysed 56 specimens using the mitochondrial gene COI. Our analysis delimits five molecular operational taxonomic units (MOTUs), and distinguishes O. impervia from O. variegata. However, we reveal important discrepancies between MOTUs and morphology-based species identification and discuss alternative hypotheses that can account for this. Finally, we indicate the need for future study that includes additional genes, and the combination of both morphology and genetic techniques (e.g. AFLP or microsatellites) to get deeper insight into species delimitation within the genus. Pensoft Publishers 2013-12-30 /pmc/articles/PMC3890686/ /pubmed/24453566 http://dx.doi.org/10.3897/zookeys.365.5356 Text en Herman Van Der Bank, Dai Herbert, Richard Greenfield, Kowiyou Yessoufou http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Van Der Bank, Herman Herbert, Dai Greenfield, Richard Yessoufou, Kowiyou Revisiting species delimitation within the genus Oxystele using DNA barcoding approach |
title | Revisiting species delimitation within the genus Oxystele using DNA barcoding approach |
title_full | Revisiting species delimitation within the genus Oxystele using DNA barcoding approach |
title_fullStr | Revisiting species delimitation within the genus Oxystele using DNA barcoding approach |
title_full_unstemmed | Revisiting species delimitation within the genus Oxystele using DNA barcoding approach |
title_short | Revisiting species delimitation within the genus Oxystele using DNA barcoding approach |
title_sort | revisiting species delimitation within the genus oxystele using dna barcoding approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890686/ https://www.ncbi.nlm.nih.gov/pubmed/24453566 http://dx.doi.org/10.3897/zookeys.365.5356 |
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