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Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis

Pseudomonas aeruginosa, is an opportunistic, bacterial pathogen causing persistent and frequently fatal infections of the lung in patients with cystic fibrosis. Isolates from chronic infections differ from laboratory and environmental strains in a range of traits and this is widely interpreted as th...

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Autores principales: Jiricny, Natalie, Molin, Søren, Foster, Kevin, Diggle, Stephen P., Scanlan, Pauline D., Ghoul, Melanie, Johansen, Helle Krogh, Santorelli, Lorenzo A., Popat, Roman, West, Stuart A., Griffin, Ashleigh S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3891558/
https://www.ncbi.nlm.nih.gov/pubmed/24454693
http://dx.doi.org/10.1371/journal.pone.0083124
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author Jiricny, Natalie
Molin, Søren
Foster, Kevin
Diggle, Stephen P.
Scanlan, Pauline D.
Ghoul, Melanie
Johansen, Helle Krogh
Santorelli, Lorenzo A.
Popat, Roman
West, Stuart A.
Griffin, Ashleigh S.
author_facet Jiricny, Natalie
Molin, Søren
Foster, Kevin
Diggle, Stephen P.
Scanlan, Pauline D.
Ghoul, Melanie
Johansen, Helle Krogh
Santorelli, Lorenzo A.
Popat, Roman
West, Stuart A.
Griffin, Ashleigh S.
author_sort Jiricny, Natalie
collection PubMed
description Pseudomonas aeruginosa, is an opportunistic, bacterial pathogen causing persistent and frequently fatal infections of the lung in patients with cystic fibrosis. Isolates from chronic infections differ from laboratory and environmental strains in a range of traits and this is widely interpreted as the result of adaptation to the lung environment. Typically, chronic strains carry mutations in global regulation factors that could effect reduced expression of social traits, raising the possibility that competitive dynamics between cooperative and selfish, cheating strains could also drive changes in P. aeruginosa infections. We compared the expression of cooperative traits - biofilm formation, secretion of exo-products and quorum sensing (QS) - in P. aeruginosa isolates that were estimated to have spent different lengths of time in the lung based on clinical information. All three exo-products involved in nutrient acquisition were produced in significantly smaller quantities with increased duration of infection, and patterns across four QS signal molecules were consistent with accumulation over time of mutations in lasR, which are known to disrupt the ability of cells to respond to QS signal. Pyocyanin production, and the proportion of cells in biofilm relative to motile, free-living cells in liquid culture, did not change. Overall, our results confirm that the loss of social behaviour is a consistent trend with time spent in the lung and suggest that social dynamics are potentially relevant to understanding the behaviour of P. aeruginosa in lung infections.
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spelling pubmed-38915582014-01-21 Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis Jiricny, Natalie Molin, Søren Foster, Kevin Diggle, Stephen P. Scanlan, Pauline D. Ghoul, Melanie Johansen, Helle Krogh Santorelli, Lorenzo A. Popat, Roman West, Stuart A. Griffin, Ashleigh S. PLoS One Research Article Pseudomonas aeruginosa, is an opportunistic, bacterial pathogen causing persistent and frequently fatal infections of the lung in patients with cystic fibrosis. Isolates from chronic infections differ from laboratory and environmental strains in a range of traits and this is widely interpreted as the result of adaptation to the lung environment. Typically, chronic strains carry mutations in global regulation factors that could effect reduced expression of social traits, raising the possibility that competitive dynamics between cooperative and selfish, cheating strains could also drive changes in P. aeruginosa infections. We compared the expression of cooperative traits - biofilm formation, secretion of exo-products and quorum sensing (QS) - in P. aeruginosa isolates that were estimated to have spent different lengths of time in the lung based on clinical information. All three exo-products involved in nutrient acquisition were produced in significantly smaller quantities with increased duration of infection, and patterns across four QS signal molecules were consistent with accumulation over time of mutations in lasR, which are known to disrupt the ability of cells to respond to QS signal. Pyocyanin production, and the proportion of cells in biofilm relative to motile, free-living cells in liquid culture, did not change. Overall, our results confirm that the loss of social behaviour is a consistent trend with time spent in the lung and suggest that social dynamics are potentially relevant to understanding the behaviour of P. aeruginosa in lung infections. Public Library of Science 2014-01-14 /pmc/articles/PMC3891558/ /pubmed/24454693 http://dx.doi.org/10.1371/journal.pone.0083124 Text en © 2014 Jiricny et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jiricny, Natalie
Molin, Søren
Foster, Kevin
Diggle, Stephen P.
Scanlan, Pauline D.
Ghoul, Melanie
Johansen, Helle Krogh
Santorelli, Lorenzo A.
Popat, Roman
West, Stuart A.
Griffin, Ashleigh S.
Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis
title Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis
title_full Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis
title_fullStr Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis
title_full_unstemmed Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis
title_short Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs of Patients with Cystic Fibrosis
title_sort loss of social behaviours in populations of pseudomonas aeruginosa infecting lungs of patients with cystic fibrosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3891558/
https://www.ncbi.nlm.nih.gov/pubmed/24454693
http://dx.doi.org/10.1371/journal.pone.0083124
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